CRYSTAL-STRUCTURE OF THE EUKARYOTIC DNA-POLYMERASE PROCESSIVITY FACTOR PCNA
CRYSTAL-STRUCTURE OF THE EUKARYOTIC DNA-POLYMERASE PROCESSIVITY FACTOR PCNA
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DOI：
10.1016/0092-8674(94)90014-0
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发表时间：
1994-12-30
期刊：
影响因子：
64.5
通讯作者：
KURIYAN, J
中科院分区：
文献类型：
--
作者：
KRISHNA, TSR;KONG, XP;KURIYAN, J
真核生物DNA聚合酶δ所需的合成因子、S.酿酒酵母，已确定在2.3埃分辨率。三个PCNA分子，每个包含两个拓扑结构相同的域，紧密相连，形成一个封闭的环。环的尺寸和静电性质表明PCNA包围双链体DNA，为聚合酶的附着提供DNA结合平台。三聚体PCNA环与大肠杆菌β亚基（持续合成因子）形成的二聚体环惊人地相似。coli DNA聚合酶III全酶，与其没有显著的序列同一性。这种结构上的对应关系进一步证实了生物化学基础上提出的真核生物和原核生物DNA复制之间的机械联系。
The crystal structure of the processivity factor required by eukaryotic DNA polymerase delta, proliferating cell nuclear antigen (PCNA) from S. cerevisiae, has been determined at 2.3 Angstrom resolution. Three PCNA molecules, each containing two topologically identical domains, are tightly associated to form a closed ring. The dimensions and electrostatic properties of the ring suggest that PCNA encircles duplex DNA, providing a DNA-bound platform for the attachment of the polymerase. The trimeric PCNA ring is strikingly similar to the dimeric ring formed by the beta subunit (processivity factor) of E. coli DNA polymerase III holoenzyme, with which it shares no significant sequence identity. This structural correspondence further substantiates the mechanistic connection between eukaryotic and prokaryotic DNA replication that has been suggested on biochemical grounds.