Biopreservation of RBCs for in vitro Plasmodium falciparum culture.
Biopreservation of RBCs for in vitro Plasmodium falciparum culture.
复制标题
用于体外恶性疟原虫培养的红细胞生物保存。
DOI：
10.1111/bjh.13882
复制
发表时间：
2016
影响因子：
6.5
通讯作者：
Cerami,Carla
中科院分区：
文献类型：
--
作者：
Goheen,MorganM;Clark,MarthaA;Kasthuri,RajS;Cerami,Carla
恶性疟疾仍然是一种毁灭性的传染病，每年造成近70万人死亡。对疟疾发病机制的进一步了解将有助于确定下一代治疗药物的分子和细胞靶点。疟疾感染的发病率和死亡率发生在红细胞期。红细胞期疟疾的研究不仅对开发新的抗疟药物至关重要，而且对提高我们对宿主病原体关系的认识也至关重要。用于培养红细胞期恶性疟原虫的方法是在30多年前开发的（詹森& Trager，1977）。然而，恶性疟原虫体外培养的优化仍在研究中。红细胞内发育受红细胞储存影响的方面以及红细胞生物保存对红细胞内生命周期的影响仍然未知。
Falciparum malaria remains a devastating infectious disease, killing nearly 700,000 people annually. Further understanding of malaria pathogenesis will help identify molecular and cellular targets of next-generation therapeutics. The morbidity and mortality of malaria infection occur during the erythrocytic stage. Study of erythrocytic stage malaria is critical not only for new anti-malarial development, but also for increasing our understanding of the host pathogen relationship. For example, many host genetic polymorphisms known to impact red blood cell (RBC) physiology also alter malaria susceptibility.Methods for culturing erythrocytic stage P. falciparum were developed more than 30 years ago (Jensen & Trager, 1977). However, optimization of in vitro P. falciparum culture is still under investigation. The aspects of intraerythrocytic development that are impacted by RBC storage, as well as the effects of RBC biopreservation on the intraerythrocytic life cycle, remain unknown.