Regenerative capacity of individual liver lobes in the microsurgical mouse model
Regenerative capacity of individual liver lobes in the microsurgical mouse model
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DOI：
10.1002/micr.20271
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发表时间：
2006-01-01
期刊：
影响因子：
2.1
通讯作者：
Candinas, Daniel
中科院分区：
文献类型：
--
作者：
Inderbitzin, Daniel;Studer, Peter;Candinas, Daniel
在小鼠肝再生和修复的显微外科模型的开发中，观察到叶特异性再生反应。因此，我们确定了单个小鼠肝叶的肝再生能力。在小鼠中，切除总肝脏质量的26%、60%、75%和83%。在收获肝脏之前注射溴脱氧尿苷（BrdU），并在所有剩余的单个肝叶中测定BrdU标记指数。BrdU阳性细胞核被认为是在所有肝叶后26和60%的切除，但显着较少检测到尾状叶。75%组BrdU阳性细胞核均匀分布，83%组BrdU阳性细胞核较少。在显微外科小鼠肝脏再生模型中，平均肝脏反应取决于切除的肝组织量和剩余的肝叶。BrdU掺入可以在各个叶之间显著变化。所观察到的叶特异性差异可能在进一步研究肝再生和修复中有价值。(c)2006 Wiley-Liss，Inc.
In the development of microsurgical mouse models of hepatic regeneration and repair, lobe-specific regenerative responses were observed. We therefore determined the hepatic regenerative capacity of individual mouse liver lobes. In mice, 26, 60, 75, and 83% of total liver mass was resected. Bromo-deoxyuridine (BrdU) was injected prior to liver harvest and the BrdU labeling index determined in all remaining individual liver lobes. BrdU-positive nuclei were seen in all liver lobes after the 26 and 60% resection, but significantly fewer were detected in the caudate lobe. In the 75% group, equally distributed positive nuclei were found. However, BrdU labeling was scant in the 83% group. In microsurgical mouse liver-regeneration models, the average hepatic response depends on amount of liver tissue resected and on the remaining liver lobe. BrdU incorporation can vary significantly among individual lobes. The lobe-specific differences observed may prove valuable in further investigations of hepatic regeneration and repair. (c) 2006 Wiley-Liss, Inc.