Cyclosporine A improves pregnancy outcomes in women with recurrent pregnancy loss and elevated Th1/Th2 ratio
Cyclosporine A improves pregnancy outcomes in women with recurrent pregnancy loss and elevated Th1/Th2 ratio
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DOI：
10.1002/jcp.28543
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发表时间：
2019-10-01
影响因子：
5.6
通讯作者：
Yousefi, Mehdi
中科院分区：
文献类型：
--
作者：
Azizi, Ramyar;Ahmadi, Majid;Yousefi, Mehdi
复发性妊娠丢失是最常见的产科并发症之一。由于成功的妊娠发生在辅助性T细胞2（Th 2）占主导地位的情况下，由于Th 1型免疫与妊娠失败有关，我们研究了环孢素对RPL妇女Th 1和Th 2细胞的影响。共纳入76例RPL患者（38例为治疗组，38例为对照组）。采用流式细胞仪检测外周血中Th 1、Th 2细胞比例。采用实时荧光定量聚合酶链反应（real-time polymerase chain reaction，RT-PCR）检测细胞内转录因子mRNA的表达，酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）检测Th 1和Th 2相关细胞因子的表达。Th 1频率（p=0.0004）、Th 1/Th 2比值（p
Recurrent pregnancy loss (RPL) is a one of the most common obstetrical complications. Since, the successful pregnancy occurs in T helper 2 (Th2)-dominant situation and since, Th1 type immunity is related to pregnancy failure, we investigated the effects of cyclosporine on Th1 and Th2 cells in RPL women. Totally, 76 RPL patients (38 women as treated group and 38 as control group) were included in this study. Flow cytometry was utilized to analyze the frequency of Th1 and Th2 in blood samples. Also, real-time polymerase chain reaction was carried out to assess the messenger RNA (mRNA) expression of transcription factors and enzyme-linked immunosorbent assay was used to evaluate Th1 and Th2 related cytokines. Significant decrease in Th1 frequency (p=0.0004), Th1/Th2 ratio (p