Identification of Mycoplasma binding proteins utilizing a 100 kilodalton lung fibroblast receptor.
Identification of Mycoplasma binding proteins utilizing a 100 kilodalton lung fibroblast receptor.
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DOI：
10.3109/10799898909066071
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
S. Geary;M. G. Gabridge;R. Intres;D. Draper;M. Gladd
中科院分区：
文献类型：
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作者：
S. Geary;M. G. Gabridge;R. Intres;D. Draper;M. Gladd
从MRC-5人肺成纤维细胞中分离到一个100千道尔顿的肺炎支原体糖蛋白受体。该受体以及抗受体血清都能够抑制14c标记的肺炎支原体与MRC-5成纤维细胞的附着。该受体还能抑制c标记的鸡毒支原体和生殖支原体对MRC-5成纤维细胞的附着，但不抑制肺支原体。这表明在肺炎支原体、生殖支原体和鸡毒支原体的这些结合蛋白中可能存在一个共同的序列。利用该受体和抗受体血清检测肺炎支原体、生殖支原体和鸡毒支原体对应的结合蛋白。在肺炎支原体、生殖支原体和鸡毒支原体中分别鉴定出一个32千道尔顿蛋白、一个90千道尔顿蛋白和一个139千道尔顿蛋白。
A 100 kilodalton glycoprotein receptor for Mycoplasma pneumoniae has been isolated from MRC-5 human lung fibroblasts. This receptor, as well as anti-receptor serum, were both capable of inhibiting the attachment of 14C-labelled M. pneumoniae to MRC-5 fibroblasts. The receptor was also capable of inhibiting the attachment of C-labelled M. gallisepticum and M. genitalium, but not M. pulmonis, to MRC-5 fibroblasts. This indicates that a common sequence may exist in these binding proteins of M. pneumoniae, M. genitalium, and M. gallisepticum. This receptor and anti-receptor serum were utilized to probe M. pneumoniae, M. genitalium, and M. gallisepticum for their corresponding binding proteins. A 32 kilodalton protein in M. pneumoniae, a 90 kilodalton protein in M. genitalium and a 139 kilodalton protein in M. gallisepticum were recognized.