Quantification of prolactin receptor mRNA in multiple human tissues and cancer cell lines by real time RT-PCR
Quantification of prolactin receptor mRNA in multiple human tissues and cancer cell lines by real time RT-PCR
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DOI：
10.1677/joe.0.171r001
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发表时间：
2001-10-01
影响因子：
4
通讯作者：
Chen, WY
中科院分区：
文献类型：
--
作者：
Peirce, SK;Chen, WY
据报道，人类催乳素（hPRL）与乳腺癌和前列腺癌的发展有关。hPRL受体（hPRLR）在多种组织中至少以三种亚型表达。本研究采用一步实时反转录PCP技术，测定了11种人乳腺癌细胞系、HeLa细胞、3种前列腺癌细胞系和9种正常人体组织中hPRLR mRNA的相对表达水平。内控基因-肌动蛋白用于内部归一化。我们证明，与正常乳腺组织相比，在11个乳腺癌细胞百合中，有6个细胞的hPRLR mRNA表达上调。在检测的癌细胞百合中，我们发现T-47D细胞的hPRLR、mRNA水平最高，其次是MDA-MB-134、BT-483、BT-474、MCF-7和MDA-MB-453细胞。在两种乳腺癌细胞系（MDA-MB-468和BT-549）中，发现hPRLR水平与正常乳腺组织相当。3种乳腺癌细胞系（MDA-MB-436、MDA-MB-157和MDA-MB-231）的hPRLR mRNA表达水平均低于正常组织。相比之下，在所有三种常用的前列腺癌细胞系（LNCaP， PC-3和DU 145）中，hPRLR， mRNA的水平相对于正常前列腺组织被发现下调。九种正常人体组织的测试。我们发现子宫和乳房的hPRLR mRNA水平最高，其次是肾脏和肝脏。前列腺和卵巢。hPRLP、mRNA水平在气管、脑和肺中最低。
Human prolactin (hPRL) has been reported to be involved in breast and prostate cancer development. The hPRL receptor (hPRLR) is expressed in a wide variety of tissues in at least three isoforms. In this study, a one-step real time reverse transcription PCP, technique was used to determine relative expression levels of hPRLR mRNA in eleven human breast cancer cell lines, HeLa cells, three prostate cancer cell lines and nine normal human tissues. The housekeeping gene beta -actin was used for internal normalization. We demonstrate that hPRLR mRNA is up-regulated in six of the eleven breast cancer cell lilies tested when compared with normal breast tissue. Of the cancer cell lilies tested, we found that T-47D cells have the highest level of hPRLR, mRNA, followed by MDA-MB-134, BT-483, BT-474, MCF-7 and MDA-MB-453 cells. In two breast cancer cell lines (MDA-MB-468 and BT-549), the hPRLR levels were found to be comparable to that of normal breast tissue. Three breast cancer cell lines (MDA-MB-436, MDA-MB-157 and MDA-MB-231) expressed hPRLR mRNA at levels lower than that of normal tissue. In contrast, in all three commonly used prostate cancer cell lines (LNCaP, PC-3 and DU 145), the levels of hPRLR, mRNA were found to be downregulated relative to that of normal prostate tissue. Of nine normal human tissues tested. we found that the uterus and the breast have the highest levels of hPRLR mRNA, followed by the kidney, the liver. the prostate and the ovary. The levels of hPRLP, mRNA were the lowest among the trachea, the brain and the lung.