Neurotoxic evaluation of two organobromine model compounds and natural AOBr-containing surface water samples by a Caenorhabditis elegans test
Neurotoxic evaluation of two organobromine model compounds and natural AOBr-containing surface water samples by a Caenorhabditis elegans test
复制标题
通过秀丽隐杆线虫测试对两种有机溴模型化合物和天然含 AOBr 地表水样进行神经毒性评估
DOI：
10.1016/j.ecoenv.2014.03.009
复制
发表时间：
2014-06-01
影响因子：
6.8
通讯作者：
Steinberg, Christian E. W.
中科院分区：
文献类型：
--
作者：
Ju, Jingjuan;Lieke, Thora;Steinberg, Christian E. W.
溴化有机化合物被称为消毒副产物。然而，最近，甚至在腐烂的淡水蓝藻水华中也发现了天然溴化有机化合物（分析为可吸附有机溴; AOBr）。在已确定的化合物中，二溴乙酸（DBAA）已被证明在哺乳动物试验中在相当高的浓度下具有神经毒性。目前，单一化合物以及复杂混合物如何在环境现实浓度下影响生物体是公开的。此外，目前还不清楚天然有机物，主要是腐殖物质（HS），这是存在于所有淡水系统，调节AOBr的毒性影响。因此，两个AOBr化合物（DBAA和四溴双酚-A; TBBP-A）和AOBr的水样品进行了测试，使用秀丽隐杆线虫神经毒性测定，测量自主神经和感觉功能。TBBP-A对线虫的三个反应变量有影响，可以归类为神经毒性。与我们的预期相反，DBAA导致两个自主神经功能的神经刺激，但对排便间隔有暂时的影响。C的暴露。在浓缩的水样中--特别是一种--增加了四种运动特征中的三种，而排便活动和两种感觉变量都没有改变。这种刺激可能是由于样品中未鉴定的化合物或AOBr化合物的激素效应。趋热行为的特征是暂时偏好较冷的环境，表明暂时的轻度神经毒性。总体而言，本研究中使用的一组相对简单的表型试验显示了对化学化合物或天然样品的有意义的神经毒性或神经刺激作用。此外，它表明，在环境现实浓度下对天然AOBr化合物的反应不一定是不利的，而是天然AOBr的混合物是神经刺激性的。(C)2014爱思唯尔公司All rights reserved.
Brominated organic compounds are known as disinfection byproducts. Very recently, however, even natural brominated organic compounds (analyzed as adsorbable organic bromine; AOBr) have been found in decaying freshwater cyanobacteria blooms. Among the identified compounds was dibromoacetic acid (DBAA), which has proven to be neurotoxic at rather high concentrations in mammalian assays. Currently it is open how single compounds as well as complex mixtures impact organisms at environmentally realistic concentrations. Furthermore, it is also unclear how natural organic matter, mainly humic substances (HS), which are present in all freshwater systems, modulates the toxic impact of AOBr. Therefore, two AOBr compounds (DBAA and tetrabromobisphenol-A; TBBP-A) and AOBr-containing water samples were tested using a Caenorhabditis elegans neurotoxicity assay that measured autonomic and sensory functions. TBBP-A had an impact on three response variables of C elegans and can be classified neurotoxic. In contrast to our expectations, DBAA led to neurostimulation of two autonomic functions, but had a temporary impact on the defecation interval.All surface water samples contained measurable amounts of AOBr. Exposure of C. elegans to concentrated water samples - one in particular - increased three of the four locomotion traits and left defecation activity and both sensory variables unchanged. This stimulation might be due to unidentified compounds in the samples or to a hormetic effect of the AOBr compounds. Thermotactic behavior was characterized by a temporary preference for the colder environment, indicating a temporary mild neurotoxicity. Overall, the set of relative simple phenotypic tests used in the current study revealed a meaningful neurotoxic or neurostimulative profile in response to chemical compounds or natural samples. Furthermore, it shows that the resulting response to natural AOBr compounds at environmentally realistic concentrations was not necessarily adverse, but instead, that the mixtures of natural AOBr were neurostimulatory. (C) 2014 Elsevier Inc. All rights reserved.