Determination of baicalein, baicalin and quercetin in Scutellariae Radix and its preparations by capillary electrophoresis with electrochemical detection
Determination of baicalein, baicalin and quercetin in Scutellariae Radix and its preparations by capillary electrophoresis with electrochemical detection
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DOI：
10.1016/s0039-9140(00)00514-2
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发表时间：
2000-11-01
期刊：
影响因子：
6.1
通讯作者：
Ye, JN
中科院分区：
文献类型：
--
作者：
Chen, G;Zhang, HW;Ye, JN
建立了毛细管电泳联用电化学检测(CE-ED)法同时测定黄芩及其制剂中黄芩素、黄芩苷和槲皮苷的含量。考察了运行缓冲液的酸度和浓度、分离电压、进样时间、工作电极电位等因素对分离效果的影响，得到了最佳分离条件。工作电极为直径300微米的碳盘电极，与毛细管出口相对。在100 mmol1(-1)的硼酸盐缓冲液(pH 9.0)中，分离电压为12kV，在40 cm长的毛细管内12min内可以很好地分离这三种分析物。三种分析物的线性范围均为3个数量级，检出限(S/N=3)为0.224~0.548 mmol1(-1)。该方法具有良好的长期稳定性和重现性，迁移时间和峰电流的相对标准偏差均小于5%(n=7)。该方法已成功应用于实际样品的分析，分析结果令人满意。(C)2000 Elsevier Science B.V.保留所有权利。
A method based on capillary electrophoresis with electrochemical detection (CE-ED) was developed for the determination of baicalein, baicalin and quercetin in Scutellariae Radix and its pharmaceutical preparations. The effects of some important factors such as the acidity and concentration of running buffer, separation voltage, injection time, and the potential of working electrode were investigated to acquire the optimum condition. The working electrode was a 300-mum diameter carbon disc electrode positioned opposite the outlet of capillary. The three analytes could be well separated within 12 min in a 40 cm length capillary at the separation voltage of 12 kV in a 100 mmol 1(-1) borate buffer (pH 9.0). The response was linear over three orders of magnitude with detection limits (S/N = 3) ranged from 0.224 to 0.548 mu mol 1(-1) for all three analytes. This proposed method demonstrated good long-term stability and reproducibility with R.S.D. of less than 5% for both migration time and peak current (n = 7). It has been successfully applied to analyze the actual samples with satisfactory assay results. (C) 2000 Elsevier Science B.V. All rights reserved.