Burns, biofilm and a new appraisal of burn wound sepsis
Burns, biofilm and a new appraisal of burn wound sepsis
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DOI：
10.1016/j.burns.2009.02.017
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发表时间：
2010-02-01
期刊：
影响因子：
2.7
通讯作者：
Wills, Edward
中科院分区：
文献类型：
--
作者：
Kennedy, Peter;Brammah, Susan;Wills, Edward
目的：烧伤后，伤口可能会定植，并可能发生脓毒性并发症。通常从烧伤伤口分离的许多生物体产生生物膜，生物膜被定义为被基质包围的表面上的生物体的集合。生物膜与抗生素抗性生物体的发展相关，并且对免疫系统是难治的。烧伤伤口中生物膜的存在尚未有记录。方法：使用光学和电子显微镜进行研究以确定烧伤伤口中生物膜的存在。使用特定的染色剂来检测微生物和相关碳水化合物（生物膜基质的主要成分）的存在。结果：在烧伤创面的溃疡区检测到生物膜。结论：烧伤创面生物被膜的发现对我们认识烧伤创面脓毒症具有重要意义，为烧伤创面早期切除和封闭提供了依据。由于生物膜相关脓毒症的难降解性和破坏生物膜的困难性，其对伤口护理敷料的未来发展具有影响。皇冠版权所有（C）2009由爱思唯尔有限公司和ISBI出版。All rights reserved.
Purpose: Following a burn, the wound may become colonized and septic complications may ensue. Many organisms, commonly isolated from burn wounds produce biofilms, which are defined as a collection of organisms on a surface surrounded by a matrix. Biofilms are associated with development of antibiotic resistant organisms and are refractory to the immune system. The presence of biofilm in the burn wound has not been documented.Methods: A study was undertaken using light and electron microscopy to determine the presence of biofilm in the burn wound. Specific stains were used to detect the presence of micro-organisms and associated carbohydrate, a major constituent of the biofilm matrix. A concurrent microbiological study of the burn wound was also carried out.Results: Biofilm was detected in ulcerated areas of the burn wound. Bacterial wound invasion with mixed organisms was also commonly detected.Conclusions: The finding of biofilm in the burn wound has significance in our understanding of burn wound sepsis and supports the evidence for early excision and closure of the burn wound. Due to the recalcitrant nature of biofilm associated sepsis and the difficulty in disrupting biofilm it has implications for the future development of wound care dressings. Crown Copyright (C) 2009 Published by Elsevier Ltd and ISBI. All rights reserved.