African Spiny Mice (Acomys) Exhibit Mild Osteoarthritis Following Meniscal Injury.
African Spiny Mice (Acomys) Exhibit Mild Osteoarthritis Following Meniscal Injury.
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DOI：
10.1177/19476035221149146
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发表时间：
2023-03
期刊：
影响因子：
2.8
通讯作者：
Allen, Kyle D.
中科院分区：
文献类型：
--
作者：
Chan, Kiara M.;Thurlow, Nat A.;Maden, Malcolm;Allen, Kyle D.
关键词：
透明软骨的先天愈合能力有限，透明软骨丧失是骨关节炎 (OA) 的一个标志。动物模型可以为软骨再生潜力提供重要的见解。其中一种动物模型是非洲刺鼠（Acomys），它能够再生皮肤、骨骼肌和弹性软骨。本研究旨在评估这些再生能力是否可以保护患有半月板损伤的 Acomys 免受 OA 相关的关节损伤以及表明关节疼痛和功能障碍的行为。 Acomys 接受了内侧半月板不稳定 (DMM) 手术 (n = 11) 或皮肤切口 (n = 10)。步态测试在术后 4、6、8、10 和 12 周进行。在终点时，对关节进行组织学处理以评估软骨损伤。关节损伤后，接受 DMM 手术的 Acomys 通过增加对侧肢体相对于手术肢体的站立时间百分比来改变他们的行走模式，从而减少受伤肢体在整个步态周期中必须自行承受重量的时间。组织学分级表明接受 DMM 手术的 Acomys 患者存在 OA 相关关节损伤的证据；这些变化主要是由于透明软骨结构完整性丧失造成的。 Acomys 出现了步态补偿，并且 Acomys 的透明软骨没有完全受到半月板损伤后与 OA 相关的关节损伤的保护，尽管这种损伤比历史上在具有相同损伤的 C57BL/6 小鼠中发现的损伤要轻。因此，尽管 Acomys 能够再生其他受伤组织，但它似乎并没有完全免受 OA 相关变化的影响。
Hyaline cartilage has limited innate healing abilities and hyaline cartilage loss is a hallmark of osteoarthritis (OA). Animal models can provide important insights into cartilage regeneration potential. One such animal model, the African spiny mouse (Acomys), is capable of regenerating skin, skeletal muscle, and elastic cartilage. This study aims to evaluate whether these regenerative abilities protect Acomys with meniscal injury from OA-related joint damage and behaviors indicative of joint pain and dysfunction. Acomys received destabilization of the medial meniscus (DMM) surgery (n = 11) or a skin incision (n = 10). Gait testing occurred at 4, 6, 8, 10, and 12 weeks after surgery. At endpoint, joints were processed for histology to assess cartilage damage. Following joint injury, Acomys with DMM surgery altered their walking patterns by increasing the percent stance time on the contralateral limb relative to the operated limb, thereby reducing the amount of time the injured limb must bear weight on its own throughout the gait cycle. Histological grading indicated evidence of OA-related joint damage in Acomys with DMM surgery; these changes were primarily driven by loss of structural integrity in the hyaline cartilage. Acomys developed gait compensations, and the hyaline cartilage in Acomys is not fully protected from OA-related joint damage following meniscal injury, although this damage was less severe than that historically found in C57BL/6 mice with an identical injury. Thus, Acomys do not appear to be completely protected from OA-related changes, despite the ability to regenerate other wounded tissues.
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影响因子：
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