Analysis of genetic diversity among natural hybrid progenies of Camellia sinensis 'Huangshanzhong'.
Analysis of genetic diversity among natural hybrid progenies of Camellia sinensis 'Huangshanzhong'.
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发表时间：
2010
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通讯作者：
F. Kai;Hong Yong-cong;Ding Zhaotang;Wang Yu
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作者：
F. Kai;Hong Yong-cong;Ding Zhaotang;Wang Yu
利用部分形态性状和ISSR标记对黄山忠茶树天然杂交后代的遗传多样性进行了研究。在形态分析的基础上，平均变异系数为20.5%(12.5%~33.2%)，Shannon编织指数平均为1.89(1.60~2.07)。利用22个ISSR引物，共扩增出402条可识别的DNA片段。其中多态信息含量(PIC)平均值为0.90，Nei‘s遗传多样性(H)和Shannon’s信息指数(I)平均值分别为0.32和0.49。结果表明，中华绒螯蟹‘黄山中’后代间遗传变异较大，形态与ISSR遗传距离呈显著正相关(r=0.61，P<0.01)。经Mantel检验，表明ISSR是进行茶树种质资源亲缘关系分析的有效工具。根据形态和ISSR数据，将几株茶树聚为一大类，只有少数单株聚为一类，这些特殊株系将对今后的茶树育种有很大的帮助。
Genetic diversity among natural hybrid progenies of Camellia sinensis ‘Huangshanzhong’ was studied,using some morphological traits and ISSR markers.The average coefficient of variation and Shannon-weave index were 20.5%(ranged between 12.5% and 33.2%)and 1.89(ranged between 1.60 and 2.07)respectively,based on investigation of morphological traits.Using 22 ISSR primers,402 discernible DNA fragments were generated,among which 396(98.5%)were polymorphic.The mean of poly-morphism information content(PIC)for the ISSR primers was 0.90.The mean of Nei's genetic diversity(H) and Shannon's information index(I) were 0.32 and 0.49 respectively,among all tested individuals.The results showed that genetic variation was high among those progenies of C.sinensis‘Huangshan-zhong’.The relationship between morphological and ISSR-based distance was significant positively correlated(r = 0.61,P = 0.01)by Mantel testing.It indicate that ISSR is a useful tool for relationship analysis of tea germplasm resources.Based on morphological and ISSR data,several plants were clusertedinto a major group,and only few individual plants were divided into single group.These special plants would be of great help to the tea breeding in future.