Evolution of transcript modification by N(6)-methyladenosine in primates.
Evolution of transcript modification by N(6)-methyladenosine in primates.
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DOI：
10.1101/gr.212563.116
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发表时间：
2017-03
期刊：
影响因子：
7
通讯作者：
White KP
中科院分区：
文献类型：
--
作者：
Ma L;Zhao B;Chen K;Thomas A;Tuteja JH;He X;He C;White KP
种群内以及密切相关的物种之间的表型差异通常是由基因表达的变异和进化驱动的。然而，大多数分析都集中在顺式调控元件（例如控制转录活性的启动子和增强子）的基因组变异的影响上，而对于转录后过程对转录物进化的影响知之甚少。 N6-甲基腺苷 (m6A) 对 RNA 的转录后修饰已被证明在整个转录组中广泛存在，这种可逆标记可以影响转录本稳定性和翻译动力学。在这里，我们分析了人类、黑猩猩和恒河猴的类淋巴母细胞系 (LCL) 中 m6A mRNA 的修饰，并确定了物种间 m6A 进化的模式。我们发现 m6A 进化与已知与调节 m6A 动力学的酶复合物相关的共有 RNA 序列基序的进化平行发生，并且 m6A 修饰基因的表达进化与 m6A 进化平行发生。
Phenotypic differences within populations and between closely related species are often driven by variation and evolution of gene expression. However, most analyses have focused on the effects of genomic variation at cis-regulatory elements such as promoters and enhancers that control transcriptional activity, and little is understood about the influence of post-transcriptional processes on transcript evolution. Post-transcriptional modification of RNA by N6-methyladenosine (m6A) has been shown to be widespread throughout the transcriptome, and this reversible mark can affect transcript stability and translation dynamics. Here we analyze m6A mRNA modifications in lymphoblastoid cell lines (LCLs) from human, chimpanzee and rhesus, and we identify patterns of m6A evolution among species. We find that m6A evolution occurs in parallel with evolution of consensus RNA sequence motifs known to be associated with the enzymatic complexes that regulate m6A dynamics, and expression evolution of m6A-modified genes occurs in parallel with m6A evolution.