Suppression of EEG visual-evoked potentials in rats through neuromodulatory focused ultrasound.
Suppression of EEG visual-evoked potentials in rats through neuromodulatory focused ultrasound.
复制标题
DOI：
10.1097/wnr.0000000000000330
复制
发表时间：
2015-03-04
期刊：
影响因子：
1.7
通讯作者：
Yoo SS
中科院分区：
文献类型：
--
作者：
Kim H;Park MY;Lee SD;Lee W;Chiu A;Yoo SS
我们研究了脉冲低强度聚焦超声(FUS)抑制光刺激引起的啮齿类动物的视觉神经反应。使用不同的声强和脉冲占空比，经颅向大鼠视皮质注射FUS。分别于超声前、中、后三次、一次、五次测量外频闪光刺激产生的视觉诱发电位(VEP)。在5%占空比(脉冲重复频率为100 Hz)和空间峰值脉冲平均声强为3W/cm2的超声作用下，VEP幅值被抑制；然而，当使用较低的声强和占空比时，这种抑制作用不存在。较高强度和占空比的应用导致VEP幅度轻微升高，提示兴奋性神经调节。我们的结果表明，脉冲FUS应用于特定区域的脑区不仅可以抑制其兴奋性，而且可以增强其兴奋性，这取决于声强和能量沉积的速度。FUS介导的神经调节的这种双峰性特征，已经通过外部声波压力波引起的神经膜电容变化的数值模型进行了预测，这表明它在神经治疗应用中具有多功能性。
We investigated the use of pulsed low-intensity focused ultrasound (FUS) to suppress the visual neural response induced by light stimulation in rodents. FUS was administered transcranially to the rat visual cortex using different acoustic intensities and pulsing duty cycles. The visual evoked potentials (VEP) generated by an external strobe light stimulation were measured three times before, once during, and five times after the sonication. The VEP magnitude was suppressed during the sonication using a 5% duty cycle (pulse-repetition frequency of 100 Hz) and spatial-peak pulse-average acoustic intensity of 3 W/cm2; however, this suppressive effect was not present when a lower acoustic intensity and duty cycle were used. The application of a higher intensity and duty cycle resulted in a slight elevation of VEP magnitude, which suggested excitatory neuromodulation. Our findings demonstrate that the application of pulsed FUS to the region-specific brain area not only suppresses its excitability, but also can enhance the excitability depending on the acoustic intensity and rate of energy deposition. This bimodal feature of FUS-mediated neuromodulation, which has been predicted by numerical models on neural membrane capacitance change by the external acoustic pressure waves, suggests its versatility for neurotherapeutic applications.