Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants
Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants
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DOI：
10.3390/plants8040095
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发表时间：
2019-04-01
期刊：
影响因子：
4.5
通讯作者：
Maruyama-Nakashita, Akiko
中科院分区：
文献类型：
--
作者：
Morikawa-Ichinose, Tomomi;Kim, Sun-Ju;Maruyama-Nakashita, Akiko
植物以硫酸盐的形式吸收硫（S），这是所有生物体必需的元素，这主要归因于拟南芥中SULTR 1;2的功能。SULTR 1;2的破坏突变体sel 1 -10具有与硫缺乏（-S）下生长的植物相似的表型。虽然-S对S代谢的影响在幼苗中得到了很好的研究，但还没有在成熟的拟南芥植物上进行研究。为了进一步研究-S对S代谢的影响，我们分析了在长日照条件下生长的野生型（WT）植物和成熟sel 1 -10的不同部位含S化合物的积累和分布。虽然sel 1 -10和WT之间的硫酸盐、半胱氨酸和谷胱甘肽的水平几乎相似，但硫代葡萄糖苷（GSL）的水平在它们之间存在差异，这取决于植物的部位。叶片和茎中的GSL水平在sel 1 -10中普遍低于WT。然而，sel 1 -10种子保持类似的水平的脂肪族GSL的野生型植物。GSL在生殖组织中的积累可能是优先考虑的，即使在sel 1 -10中硫酸盐供应有限，因为它在S储存和植物防御中的作用。
Plants take up sulfur (S), an essential element for all organisms, as sulfate, which is mainly attributed to the function of SULTR1;2 in Arabidopsis. A disruption mutant of SULTR1;2, sel1-10, has been characterized with phenotypes similar to plants grown under sulfur deficiency (-S). Although the effects of -S on S metabolism were well investigated in seedlings, no studies have been performed on mature Arabidopsis plants. To study further the effects of -S on S metabolism, we analyzed the accumulation and distribution of S-containing compounds in different parts of mature sel1-10 and of the wild-type (WT) plants grown under long-day conditions. While the levels of sulfate, cysteine, and glutathione were almost similar between sel1-10 and WT, levels of glucosinolates (GSLs) differed between them depending on the parts of the plant. GSLs levels in the leaves and stems were generally lower in sel1-10 than those in WT. However, sel1-10 seeds maintained similar levels of aliphatic GSLs to those in WT plants. GSL accumulation in reproductive tissues is likely to be prioritized even when sulfate supply is limited in sel1-10 for its role in S storage and plant defense.