Application of infrared laser to living cells for manipulation of gene expression, and in vivo temperature measurement method
Application of infrared laser to living cells for manipulation of gene expression, and in vivo temperature measurement method
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红外激光应用于活细胞操纵基因表达以及体内温度测量方法
DOI：
10.1117/12.2615414
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kamei Yasuhiro
中科院分区：
文献类型：
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作者：
Tomoi Takumi;Sakamoto Joe;Ohe Suguru;Tamada Yosuke;Kamei Yasuhiro
红外激光通过显微镜光学加热和细胞的热休克反应相结合，实现了体内单细胞基因诱导。我们将介绍活细胞操作技术，并展示活动物和植物的代表性数据。此外，我们将讨论通过使用荧光温度计的温度成像技术在体内的局部加热性能。
Combination of infrared laser heating through a microscope optics and heat shock response of cells achieves single-cell gene induction in vivo. We will introduce a live-cell manipulation technique and show representative data in living animals and plants. Further, we will discuss a local heating property in vivo through a temperature imaging technique using a fluorescent thermometer.