Use of genome selected repeated sequences increases the sensitivity of PCR detection of Tropheryma whipplei
Use of genome selected repeated sequences increases the sensitivity of PCR detection of Tropheryma whipplei
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DOI：
10.1128/jcm.42.1.401-403.2004
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发表时间：
2004-01-01
影响因子：
9.4
通讯作者：
Raoult, D
中科院分区：
文献类型：
--
作者：
Fenollar, F;Fournier, PE;Raoult, D
惠氏巨瘤基因组的可用性为选择逻辑DNA靶标提供了假定的可能性。我们对惠普尔病患者和对照组的样本进行了PCR检测（针对惠普尔氏杆菌的重复序列）。与常规PCR相比，重复PCR的敏感性显著提高（P = 0.02），但特异性没有改变。
The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with regular PCR, the sensitivity of repeat PCR was significantly enhanced (P = 0.02) without alteration of its specificity.