BOTH THE V2 AND V3 REGIONS OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 SURFACE GLYCOPROTEIN FUNCTIONALLY INTERACT WITH OTHER ENVELOPE REGIONS IN SYNCYTIUM FORMATION
BOTH THE V2 AND V3 REGIONS OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 SURFACE GLYCOPROTEIN FUNCTIONALLY INTERACT WITH OTHER ENVELOPE REGIONS IN SYNCYTIUM FORMATION
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DOI：
10.1128/jvi.67.6.3232-3239.1993
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发表时间：
1993-06-01
影响因子：
5.4
通讯作者：
BOSCH, ML
中科院分区：
文献类型：
--
作者：
ANDEWEG, AC;LEEFLANG, P;BOSCH, ML
要映射的区域参与膜融合的过程中的人类免疫缺陷病毒1型（HIV-1）的外部包膜糖蛋白，我们确定了合胞体诱导能力的一个面板的瞬时表达嵌合包膜基因。该面板是通过在先前研究的四个包膜基因之间交换基因片段而产生的，所述包膜基因表现出高度的序列同源性，但当由重组牛痘病毒表达时，在合胞体诱导能力方面显示出显著差异。结果表明，HIV-1包膜糖蛋白的多个区域参与合胞体的形成。一些片段，最值得注意的是那些含有V2或V3区，可以转移合胞体诱导能力的包膜蛋白以前不能诱导合胞体。此外，它表明，这样的区域功能性地与其他包膜区域，特别是一个涵盖的V4和V5区的gp 120或一个区域涵盖的gp 41的一部分，发挥其功能，在膜融合。
To map the regions of the external envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) involved in the process of membrane fusion, we determined the syncytium-inducing capacity of a panel of transiently expressed chimeric envelope genes. This panel was generated by exchanging gene fragments between four previously studied envelope genes that exhibited a high degree of sequence homology yet displayed marked differences in syncytium-inducing capacity when expressed by recombinant vaccinia virus. The results demonstrate that multiple regions of the HIV-1 envelope glycoproteins are involved in syncytium formation. Some fragments, most notably those containing the V2 or V3 region, can transfer syncytium-inducing capacity to envelope proteins previously not capable of inducing syncytia. Moreover, it is shown that such regions functionally interact with other envelope regions, especially one encompassing the V4 and V5 regions of gp120 or a region encompassing part of gp41, to exert their function in membrane fusion.