Functionalization of magnetic gold/iron-oxide composite nanoparticles with oligonucleotides and magnetic separation of specific target
Functionalization of magnetic gold/iron-oxide composite nanoparticles with oligonucleotides and magnetic separation of specific target
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DOI：
10.1016/j.jmmm.2006.10.1161
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发表时间：
2007-04
影响因子：
2.7
通讯作者：
T. Kinoshita;S. Seino;Y. Mizukoshi;T. Nakagawa;T. Yamamoto
中科院分区：
文献类型：
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作者：
T. Kinoshita;S. Seino;Y. Mizukoshi;T. Nakagawa;T. Yamamoto
利用伽马射线或超声波合成的金-氧化铁磁性复合纳米粒子，用硫醇修饰的寡核苷酸进行功能化处理。用荧光标记的目标寡核苷酸定量检测通过杂交与功能化纳米颗粒探针结合的寡核苷酸的量。我们的复合纳米颗粒可以磁性分离特定的目标寡核苷酸，而不存在非特异性吸附。
Magnetic composite nanoparticles of gold and iron-oxide synthesized with gamma-rays or ultrasonics were functionalized with thiol-modified oligonucleotides. The amount of oligonucleotides bound to the functionalized nanoparticle probes via hybridization was quantified with fluorescently-labeled target oligonucleotides. Our composite nanoparticles magnetically separated the specific target oligonucleotides without the non-specific adsorption.