Environment-Sensitive Fluorescent Probe: A Benzophosphole Oxide with an Electron-Donating Substituent
Environment-Sensitive Fluorescent Probe: A Benzophosphole Oxide with an Electron-Donating Substituent
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DOI：
10.1002/anie.201500229
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发表时间：
2015-04-07
影响因子：
16.6
通讯作者：
Yamaguchi, Shigehiro
中科院分区：
文献类型：
--
作者：
Yamaguchi, Eriko;Wang, Chenguang;Yamaguchi, Shigehiro
给电子基连接在接受电子的苯并膦骨架上。在所制备的几种衍生物中，一种苯并膦氧化物尤其令人感兴趣，因为它即使在极性和质子性溶剂中也能保持高的荧光量子产率。这种磷基化合物的荧光光谱随着溶剂的极性发生了剧烈的颜色变化，而吸收光谱几乎保持不变。利用这些特征，这种基于磷素的化合物被用于对脂肪细胞进行染色，然后可以根据荧光发射的颜色变化来区分亚细胞隔间的极性。
Electron-donating aryl groups were attached to electron-accepting benzophosphole skeletons. Among several derivatives thus prepared, one benzophosphole oxide was particularly interesting, as it retained high fluorescence quantum yields even in polar and protic solvents. This phosphole-based compound exhibited a drastic color change of its fluorescence spectrum as a function of the solvent polarity, while the absorption spectra remained virtually unchanged. Capitalizing on these features, this phosphole-based compound was used to stain adipocytes, in which the polarity of subcellular compartments could then be discriminated on the basis of the color change of the fluorescence emission.