Feline aminopeptidase N is not a functional receptor for avian infectious bronchitis virus.
Feline aminopeptidase N is not a functional receptor for avian infectious bronchitis virus.
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猫氨基肽酶N不是禽传染性支气管炎病毒的功能受体。
DOI：
10.1186/1743-422x-4-20
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发表时间：
2007-02-26
期刊：
影响因子：
4.8
通讯作者：
Whittaker, Gary R.
中科院分区：
文献类型：
--
作者：
Chu, Victor C.;McElroy, Lisa J.;Aronson, Jed M.;Oura, Trisha J.;Harbison, Carole E.;Bauman, Beverley E.;Whittaker, Gary R.
冠状病毒是包括严重急性呼吸综合征(SARS)在内的人类传染病的重要原因，并具有从动物物种中出现的持续潜力。冠状病毒宿主范围中的一个主要因素是它在宿主细胞上的受体利用。在许多情况下，冠状病毒与受体的相互作用是众所周知的。然而，一个值得注意的例外是第3组冠状病毒对受体的利用，包括鸡传染性支气管炎病毒(IBV)。猫氨肽酶N(FAPN)是大多数第1组冠状病毒的功能性受体，包括猫传染性腹膜炎病毒(FIPV)、犬冠状病毒、传染性胃肠炎病毒(TGEV)和人类冠状病毒229E(HCoV-229E)。最近的一份报告也提出了fAPN在IBV进入过程中的作用(Miguel B，Pharr GT，Wang C：猫氨基肽酶N作为传染性支气管炎病毒受体的作用)。简要回顾。Arch Virol 2002,147：2047-2056。在这里，我们表明，尽管在BHK-21细胞上瞬时转染和组成性表达fAPN都可以挽救非许可BHK细胞中的FIPV和TGEV感染，但fAPN的表达不能挽救原型IBV Mass41株的感染。为了解释先前关于fAPN可能作为IBV受体的建议，我们证明了猫细胞可以被IBV原型毒株(质量41)感染，但与原代鸡肾细胞相比敏感性较低。我们还发现BHK-21细胞对某些IBV毒株包括Ark99、Ark_DPI、CA99和IOWA97(效率为0.01%)有轻微的敏感性，但这种感染水平不会因fAPN的表达而增加。我们得出结论，fAPN不是IBV的功能性受体，其特性目前正在研究中。
Coronaviruses are an important cause of infectious diseases in humans, including severe acute respiratory syndrome (SARS), and have the continued potential for emergence from animal species. A major factor in the host range of a coronavirus is its receptor utilization on host cells. In many cases, coronavirus-receptor interactions are well understood. However, a notable exception is the receptor utilization by group 3 coronaviruses, including avian infectious bronchitis virus (IBV). Feline aminopeptidase N (fAPN) serves as a functional receptor for most group 1 coronaviruses including feline infectious peritonitis virus (FIPV), canine coronavirus, transmissible gastroenteritis virus (TGEV), and human coronavirus 229E (HCoV-229E). A recent report has also suggested a role for fAPN during IBV entry (Miguel B, Pharr GT, Wang C: The role of feline aminopeptidase N as a receptor for infectious bronchitis virus. Brief review. Arch Virol 2002, 147:2047–2056. Here we show that, whereas both transient transfection and constitutive expression of fAPN on BHK-21 cells can rescue FIPV and TGEV infection in non-permissive BHK cells, fAPN expression does not rescue infection by the prototype IBV strain Mass41. To account for the previous suggestion that fAPN could serve as an IBV receptor, we show that feline cells can be infected with the prototype strain of IBV (Mass 41), but with low susceptibility compared to primary chick kidney cells. We also show that BHK-21 cells are slightly susceptible to certain IBV strains, including Ark99, Ark_DPI, CA99, and Iowa97 (<0.01% efficiency), but this level of infection is not increased by fAPN expression. We conclude that fAPN is not a functional receptor for IBV, the identity of which is currently under investigation.