Curcumin attenuates cyclooxygenase-2 expression via inhibition of the NF-B pathway in lipopolysaccharide-stimulated human gingival fibroblasts
Curcumin attenuates cyclooxygenase-2 expression via inhibition of the NF-B pathway in lipopolysaccharide-stimulated human gingival fibroblasts
复制标题
DOI：
10.1002/cbin.10050
复制
发表时间：
2013-05-01
影响因子：
3.9
通讯作者：
Chen, Min Wei
中科院分区：
文献类型：
--
作者：
Hu, Ping;Huang, Ping;Chen, Min Wei
牙龈卟啉单胞菌脂多糖（LPS）诱导环加氧酶-2（考克斯-2）的表达，从而促进牙周炎的发生。姜黄素是姜黄的一种成分，具有抗炎特性。我们研究了姜黄素对牙龈卟啉单胞菌LPS刺激的人牙龈成纤维细胞（HGFs）的抗炎作用及其作用机制。用牙龈卟啉单胞菌LPS刺激用姜黄素预处理的HGFs。实时荧光定量PCR和Western blot分析考克斯-2 mRNA和蛋白表达。核因子-B（NF-B）的激活通过NF-B依赖的荧光素酶活性和电泳迁移率变动分析（EMSA）来分析。姜黄素以剂量依赖性方式抑制LPS刺激的HGF中考克斯-2 mRNA和蛋白的合成。牙龈卟啉单胞菌LPS激活HGF中的NF-B依赖性转录，其也通过姜黄素预处理下调。因此，姜黄素能够抑制牙龈卟啉单胞菌LPS诱导的考克斯-2表达，其机制可能与抑制NF-B通路有关。
Porphyromonas gingivalis lipopolysaccharide (LPS) induces the expression of the cyclooxygenase-2 (COX-2), which contributes to the process of periodontitis. Curcumin, a constituent of turmeric, exhibits anti-inflammatory properties. We have investigated the anti-inflammatory effect of curcumin in human gingival fibroblasts (HGFs) stimulated by P. gingivalis LPS and its mechanism of action. HGFs pretreated with curcumin were stimulated by P. gingivalis LPS. COX-2 mRNA and protein expressions were analysed by real-time PCR and Western blot analysis. Activation of nuclear factor kappa B (NF-B) was analysed by the NF-B-dependent luciferase activity and electrophoretic mobility-shift assay (EMSA). Curcumin inhibited COX-2 mRNA and protein synthesis in LPS-stimulated HGFs in a dose-dependent manner. P. gingivalis LPS activated NF-B-dependent transcription in HGFs, which were also downregulated by pretreatment with curcumin. Therefore, curcumin can inhibit P. gingivalis LPS-induced COX-2 expression, which may be due to the inhibition of the NF-B pathway.