The immunoglobulin G subclass distribution of anti-GBM autoantibodies against rHα3(IV)NC1 is associated with disease severity
The immunoglobulin G subclass distribution of anti-GBM autoantibodies against rHα3(IV)NC1 is associated with disease severity
复制标题
针对 rHalpha3(IV)NC1 的抗 GBM 自身抗体的免疫球蛋白 G 亚类分布与疾病严重程度相关。
DOI：
10.1016/j.humimm.2009.04.004
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发表时间：
2009-06-01
期刊：
影响因子：
2.7
通讯作者：
Zhao, Ming-Hui
中科院分区：
文献类型：
--
作者：
Zhao, Juan;Yan, Yu;Zhao, Ming-Hui
本研究旨在探讨抗肾小球基底膜(GBM)、免疫球蛋白(Ig)G亚类分布与人类抗GBM肾炎严重程度的关系。将从5名健康献血员体内纯化的天然抗GBM-Ig G亲和力定义为A组。73例中国抗-GBM病患者根据其诊断的血肌酐(Scr)水平分为B、C、D三组。用重组人α3链非胶原区1[rhα3(IV)NC1]酶联免疫吸附试验检测抗GBM Ig G亚类。抗-GBM-Ig G I在A组(0%)未检出，B组(7.7%)出现，C组(69%)显著高于C组(69%)，D组最高(93.5%)。C、D两组IgG3阳性率明显高于A、B两组，A组抗GBM抗体亚类分布仅限于IgG4、IgG2，B组以IgG2、IgG4为主，可检出IgGI型：C组以IgGI型为主，可检出IgG3；结论：抗GBM自身抗体Ig G亚类分布与疾病严重程度相关。Ig G I和Ig G3可能在该病的发生和发展中起重要作用。(C)2009年，由埃尔塞维尔石油公司代表美国组织相容性和免疫遗传学会出版。
The current Study aimed to investigate the association between antiglomerular basement membrane (GBM) immunoglobulin (Ig) G subclass distribution and the severity of anti-GBM nephritis in human beings. Natural anti-GBM IgG affinity purified from five healthy donors was defined as group A. A total of 73 Chinese patients with anti-GBM disease were divided into three groups (groups B, C, and D) according to their serum creatinine (Scr) on diagnosis. Anti-GBM IgG Subclasses were detected by enzyme-linked immunosorbent assay using recombinant human noncollagenous domain 1 of type IV collagen of the alpha 3 chain [rH alpha 3(IV)NC1]. Anti-GBM IgG I Could not be detected in group A (0%), appeared in group B (7.7%), was significantly higher in group C (69%), and was the highest in group D (93.5%). The frequency of IgG3 ill groups C and D was significantly higher than that in groups A and B. In group A, anti-GBM IgG subclass distribution was restricted to IgG4 and IgG2; in group B, IgG2 and IgG4 remained the Major Subclasses with detectable IgG I : in group C, IgG I became predominant and IgG3 was detectable; ill group D, four IgG Subclasses Could be detected. Ill conclusion, anti-GBM autoantibody IgG subclass distribution was associated with disease severity. IgG I and IgG3 may play a major role in the initiation and progression of the disease. (C) 2009 Published by Elsevier Inc. oil behalf of American Society for Histocompatibility and Immunogenetics.