Making Waves: Initiation and Propagation of Corticothalamic Ca2+ Waves In Vivo
Making Waves: Initiation and Propagation of Corticothalamic Ca2+ Waves In Vivo
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DOI：
10.1016/j.neuron.2013.01.031
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发表时间：
2013-03-20
期刊：
影响因子：
16.2
通讯作者：
Konnerth, Arthur
中科院分区：
文献类型：
--
作者：
Stroh, Albrecht;Adelsberger, Helmuth;Konnerth, Arthur
皮质丘脑神经元活动的缓慢振荡决定内部大脑状态。至少在皮质中，电活动与大的神经元Ca 2+瞬变相关。在这里，我们实施了一种光遗传学的方法来探索在体内小鼠大脑中产生慢振荡相关的Ca 2+波的因果特征。我们证明，短暂的光遗传刺激（3-20毫秒）的局部组的第5层皮层神经元是足够的全球脑钙波的诱导。这些Ca 2+波以全或无的方式诱发，在重复刺激期间表现出不应性，并长距离传播。通过局部光遗传学刺激，我们证明了诱发的Ca 2+波最初侵入皮层，其次是丘脑的二次招募。总之，我们的研究结果表明，在一小群第5层皮层神经元的同步活动可以启动一个全球性的神经元波的活动，适合长距离皮质丘脑整合。
Corticothalamic slow oscillations of neuronal activity determine internal brain states. At least in the cortex, the electrical activity is associated with large neuronal Ca2+ transients. Here we implemented an optogenetic approach to explore causal features of the generation of slow oscillation-associated Ca2+ waves in the in vivo mouse brain. We demonstrate that brief optogenetic stimulation (3-20 ms) of a local group of layer 5 cortical neurons is sufficient for the induction of global brain Ca2+ waves. These Ca2+ waves are evoked in an all-or-none manner, exhibit refractoriness during repetitive stimulation, and propagate over long distances. By local optogenetic stimulation, we demonstrate that evoked Ca2+ waves initially invade the cortex, followed by a secondary recruitment of the thalamus. Together, our results establish that synchronous activity in a small cluster of layer 5 cortical neurons can initiate a global neuronal wave of activity suited for long-range corticothalamic integration.