Measurement of the CD3− 4+ Variant T Cell Frequency by Flow Cytometry after X‐Irradiation on Mice
Measurement of the CD3− 4+ Variant T Cell Frequency by Flow Cytometry after X‐Irradiation on Mice
复制标题
小鼠 X 射线照射后通过流式细胞术测量 CD3−4+ 变异 T 细胞频率
DOI：
10.1539/joh.38.25
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发表时间：
1996
影响因子：
3
通讯作者：
T. Norimura
中科院分区：
文献类型：
--
作者：
N. Kunugita;N. Mei;S. Nomoto;T. Norimura
流式细胞术检测X射线照射后小鼠CD_3、−_4~+变异型T细胞频率日本职业与环境卫生大学医学院放射生物学与卫生学系-用流式细胞仪检测T细胞受体(TCR)基因表达缺陷小鼠T淋巴细胞的变异细胞率。照射后取小鼠脾T淋巴细胞，分别用荧光素标记的抗L 3T4抗体和藻红蛋白标记的抗CD3-GB抗体进行染色。用流式细胞仪检测TcR/CD3复合体表面表达缺失的变异T细胞，显示CD3−4+T细胞。3Gy射线照射后3d可见变异T细胞。3GyBALB/c小鼠受照后15~21d，T细胞变异率最高达94×10−4，随后逐渐下降，半衰期约为17d。用该方法检测了BALB/c、C57BL/6、C3H/He和B6C3F1小鼠T淋巴细胞辐射敏感性的品系差异。我们观察到BALB/c表现出最高的放射敏感性。与其他方法相比，该方法快速、简便，可用于致突变性检测。
Measurement of the CD3− 4+ Variant T Cell Frequency by Flow Cytometry after X‐ Irradiation on Mice: Naoki Kunugita, et al. Department of Radiation Biology and Health, School of Medicine, University of Occupational and Environmental Health, Japan—Variant cell frequency of mice T‐lymphocytes defective in the T‐cell receptor (TCR) gene expression was measured by flow cytometry. Splenic T‐lymphocytes were obtained from mice after irradiation and stained with fluorescein‐labeled anti‐L 3 T 4 (CD 4) and phycoerythrin‐ labeled anti‐CD3‐£ antibodies. They were analyzed with a flow cytometer to detect variant T cells lacking surface expression of TCR/CD 3 complexes which showed CD3− 4+ T cells. Variant T cells could be observed 3 days after 3 Gy irradiation. Frequency of variant T cells increased to the maximum level of 94×10−4 between 15 and 21 days after 3 Gy irradiation on BALB/c mice and gradually decreased with a half‐life of approximately 17 days. We examined the strain difference in radiosensitivity of splenic T‐lymphocytes in BALB/c, C57BL/6, C3H/ He and B6C3F1 mouse strains by this assay method. We observed that BALB/c showed the most radiosensitivity. This assay method is quick and easy in comparison with other methods and is considered useful for the mutagenicity test.