Microfeature guided skeletal muscle tissue engineering for highly organized 3-dimensional free-standing constructs
Microfeature guided skeletal muscle tissue engineering for highly organized 3-dimensional free-standing constructs
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DOI：
10.1016/j.biomaterials.2008.11.014
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发表时间：
2009-02-01
期刊：
影响因子：
14
通讯作者：
Takayama, Shuichi
中科院分区：
文献类型：
--
作者：
Lam, Mai T.;Huang, Yen-Chih;Takayama, Shuichi
在结构上与其天然等同物相似的工程组织是一项重大挑战，对功能至关重要。尽管试图工程化骨骼肌，但仍然难以有效地模仿组织结构。刚性支架可以引导细胞排列，但具有阻碍所得组织的机械功能的关键缺点。我们提出了一种方法，用于创建高度有序的组织，只有通过使用刚性microtopographically图案化的表面，首先引导成肌细胞对齐，然后通过对齐的肌管转移到一个可降解的水凝胶和自组织的有序细胞成一个功能，3维，独立的结构，独立于初始模板基板。组织学显示细胞内组织类似于天然肌肉。与对照相比，对齐的细胞构建体表现出峰值力产生的2倍增加。有效比力或在横截面积上归一化的力增加了23%。这种模板、转移和自组织策略被设想为在改善高度有序的组织如肌肉的构建功能和临床适用性方面广泛有用。(C)2008爱思唯尔有限公司保留所有权利。
Engineering tissue similar in structure to their natural equivalents is a major challenge and crucial to function. Despite attempts to engineer skeletal muscle, it is still difficult to effectively mimic tissue architecture. Rigid scaffolds can guide cell alignment but have the critical drawback of hindering mechanical function of the resultant tissue. We present a method for creating highly ordered tissue-only constructs by using rigid rnicrotopographically patterned surfaces to first guide myoblast alignment, followed by transfer of aligned myotubes into a degradable hydrogel and self-organization of the ordered cells into a functional, 3-dimensional, free-standing construct independent of the initial template substrate. Histology revealed an intracellular organization resembling that of native muscle. Aligned cell constructs exhibited a 2-fold increase in peak force production compared to controls. Effective specific force, or force normalized over cross-sectional area, was increased by 23%. This template, transfer, and self-organization strategy is envisioned to be broadly useful in improving construct function and clinical applicability for highly ordered tissues like muscle. (C) 2008 Elsevier Ltd. All rights reserved.