Dietary conjugated linoleic acid modulates morphology, selective immune parameters, and gene expressions in the intestine of grass carp
Dietary conjugated linoleic acid modulates morphology, selective immune parameters, and gene expressions in the intestine of grass carp
复制标题
日粮共轭亚油酸调节草鱼肠道形态、选择性免疫参数和基因表达
DOI：
10.1016/j.fsi.2018.11.071
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发表时间：
2019-03-01
影响因子：
4.7
通讯作者：
Dong, Gui-Fang
中科院分区：
文献类型：
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作者：
Kong, Long;Cheng, Shi-yan;Dong, Gui-Fang
共轭亚油酸(CLA)已被证明在哺乳动物的肠道中具有抗炎特性。然而，共轭亚油酸对鱼类肠道免疫反应的影响尚不清楚。为此，进行了65d的生长试验，研究了饲料中添加共轭亚油酸(CLA)对草鱼肠道形态、选择性免疫参数和基因表达的影响。每100g饲料中添加0(对照)、0.5(CLA0.5)、1(CLA1)、1.5(CLA1.5)、2(CLA2)、2.5(CLA2.5)和3(CLA3)g共轭亚油酸。补体3(C3)和免疫球蛋白M(IgM)含量在添加CLA1.5~CLA2.5组显著高于对照组(P<；0.05)。CLA1.5~CLA2.5显著增加PI、MI和DI中抗炎细胞因子(IL-10和转化生长因子β1)的基因表达水平，显著降低促炎症细胞因子(IL-Lβ、IL-8和肿瘤坏死因子-α)的基因表达水平(P<；0.05)。这种促进草鱼肠道抗炎细胞因子表达和抑制促炎细胞因子表达的作用，可能是通过TLR4/NF-kappa B信号通路来实现的。我们的结果表明，CLA1.5-CLA2饲料改善了草鱼肠道的形态，增加了抗炎细胞因子的表达，抑制了促炎细胞因子的表达。综上所述，饲料中添加1.5%~2%的CIA对草鱼肠道具有抗炎治疗作用。CIA的抗炎治疗作用可能是通过TLR4/NF-kappaB信号通路实现的。
Conjugated linoleic acid (CLA) has been shown to exhibit anti-inflammatory properties in the intestine in mammals. However, the effect of CLA on intestinal immune response in fish is still unknown. Therefore, a 65-day growth trial was conducted to investigate the effects of dietary conjugated linoleic acid (CLA) on morphology, selective immune parameters, and gene expressions in the intestine of grass carp. Seven isonitrogenous and isolipidic diets were formulated as follows: 0 (control), 0.5 (CLA0.5), 1 (CLA1), 1.5 (CLA1.5), 2 (CLA2), 2.5 (CLA2.5), and 3 (CLA3) g CLA per 100g of feed.Results: showed that dietary supplementation of 1.5-3% CLA significantly (P < 0.05) increased the fold and enterocyte heights in the PI and MI of grass carp. Complement 3 (C3) and immunoglobulin M (IgM) contents in three intestinal segments were significantly (P < 0.05) higher in fish fed with CLA1.5 to CLA2.5 diets compared to fish fed the control diet. CLA1.5 to CLA2.5 diets significantly (P < 0.05) increased the mRNA expression levels of anti-inflammatory cytokines (IL-10 and TGF beta 1) and significantly (P < 0.05) reduced the mRNA expression levels of pro-inflammatory cytokines (IL-l beta, IL-8, and TNF-alpha) in the PI, MI, and DI. This improved expression of anti-inflammatory cytokines and the inhibited expression of pro-inflammatory cytokines in the intestine of grass carp, might be mediated via TLR4/NF-kappa B-signaling pathway. Our results suggested that CLA1.5 to CLA2 diets improved intestinal morphology, increased the expression of anti-inflammatory cytokines, and inhibited the expression of pro-inflammatory cytokines in the intestine of grass carp. In conclusion, dietary supplementation of 1.5%-2% CIA show the anti-inflammatory therapeutic potential in the intestine of grass carp. The anti-inflammatory therapeutic potential of CIA might be mediated via TLR4/NF-kappa B-signaling pathway.