Polarized distribution of Listeria monocytogenes surface protein ActA at the site of directional actin assembly.
Polarized distribution of Listeria monocytogenes surface protein ActA at the site of directional actin assembly.
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发表时间：
1993-07
影响因子：
4
通讯作者：
Christine Kocks;Raymond Hellio;Pierre Gounon;Hélène Ohayon;Pascale Cossart
中科院分区：
文献类型：
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作者：
Christine Kocks;Raymond Hellio;Pierre Gounon;Hélène Ohayon;Pascale Cossart
兼性细胞内病原体单核细胞增生李斯特氏菌可以通过使用定向肌动蛋白组装将自身从一个细胞推进到另一个细胞来感染宿主组织。这种运动是由肌动蛋白从细菌一端连续组装到尾巴中产生的，尾巴留在细胞质中。细菌肌动蛋白组装需要细菌基因 actA 的表达。我们使用免疫细胞化学表明，actA 基因产物 ActA 在细菌表面不对称分布：它不在一个极表达，而是越来越集中到另一极。 ActA 的这种极化分布与细菌分裂有关：ActA 蛋白在上一次分裂过程中形成的极点上没有或只有微弱的表达。在细胞内细菌上，ActA 在肌动蛋白组装位点表达，表明 ActA 可能参与肌动蛋白丝从细菌表面成核。我们预测这种蛋白质的不对称分布是细胞内李斯特菌向非 ActA 表达极方向移动的能力所必需的。
The facultative intracellular pathogen Listeria monocytogenes can infect host tissues by using directional actin assembly to propel itself from one cell into another. The movement is generated by continuous actin assembly from one end of the bacterium into a tail, which is left behind in the cytoplasm. Bacterial actin assembly requires expression of the bacterial gene actA. We have used immunocytochemistry to show that the actA gene product, ActA, is distributed asymmetrically on the bacterial surface: it is not expressed at one pole and is increasingly concentrated towards the other. This polarized distribution of ActA was linked to bacterial division: ActA protein was not, or only faintly, expressed at the pole that had been formed during the previous division. On intracellular bacteria ActA was expressed at the site of actin assembly, suggesting that ActA may be involved in actin filament nucleation off the bacterial surface. We predict that the asymmetrical distribution of this protein is required for the ability of intracellular Listeria to move in the direction of the non-ActA expressing pole.