Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits
Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits
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DOI：
10.1038/ncb837
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发表时间：
2002-09-01
影响因子：
21.3
通讯作者：
Almers, W
中科院分区：
文献类型：
--
作者：
Merrifield, CJ;Feldman, ME;Almers, W
作为内吞作用的最后一步，网格蛋白包被小窝必须从质膜上分离，并作为包被囊泡进入细胞质。由于这些过程涉及的微小运动是常规光学显微镜无法分辨的，所以它们尚未被直接观察到，其动力学也仍未被探究。在此，我们利用消逝场显微镜观察单个网格蛋白包被小窝或囊泡从质膜向内凹陷并最终脱去包被的过程。这种向内运动在发动蛋白短暂聚集爆发之后立即发生，并伴有短暂的肌动蛋白组装。因此，发动蛋白可能提供触发机制，而肌动蛋白可能为进入细胞质的运动提供动力。
As a final step in endocytosis, clathrin-coated pits must separate from the plasma membrane and move into the cytosol as a coated vesicle. Because these events involve minute movements that conventional light microscopy cannot resolve, they have not been observed directly and their dynamics remain unexplored. Here, we used evanescent field (EF) microscopy to observe single clathrin-coated pits or vesicles as they draw inwards from the plasma membrane and finally lose their coats. This inward movement occurred immediately after a brief burst of dynamin recruitment and was accompanied by transient actin assembly. Therefore, dynamin may provide the trigger and actin may provide the force for movement into the cytosol.