DEMONSTRATION OF CROSS-REACTIVE ANTIBODIES TO SMOOTH GRAM-NEGATIVE BACTERIA IN ANTISERUM TO ESCHERICHIA-COLI J5
DEMONSTRATION OF CROSS-REACTIVE ANTIBODIES TO SMOOTH GRAM-NEGATIVE BACTERIA IN ANTISERUM TO ESCHERICHIA-COLI J5
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DOI：
10.1093/infdis/156.1.136
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发表时间：
1987-07-01
影响因子：
6.4
通讯作者：
ZIEGLER, EJ
中科院分区：
文献类型：
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作者：
BAUMGARTNER, JD;OBRIEN, TX;ZIEGLER, EJ
我们研究了大肠杆菌J5抗血清对革兰氏阴性菌感染的广泛交叉保护作用与其体外明显狭窄的交叉反应性之间的差异。用J5细菌免疫的兔产生针对J5脂多糖（LPS;通过ELISA的滴度，1：60，000）和来自沙门氏菌的Re突变体（即，酮脱氧辛酸[KDO]和脂质A决定簇;滴度，1：3，200）。在高度稀释的抗血清中，经7株光滑革兰阴性菌吸附后，抗J5 LPS抗体滴度下降28%~ 41%，而经Re突变体吸附后仅下降4%。光滑的革兰氏阴性菌吸附几乎所有的抗体Re LPS。因此，兔抗J5血清含有KDO远端核心决定簇的类型特异性抗体，其可在体外掩盖脂质A-KDO的高度交叉反应性抗体。通过在有限抗体浓度下与全细菌的吸附可以证明交叉反应性抗体。
We investigated the discrepancy between the broad cross-protection against gram-negative infections afforded by antiserum to Escherichia coli J5 and its apparently narrow cross-reactivity in vitro. Rabbits immunized with J5 bacteria produced antibodies to both the J5 lipopolysaccharide (LPS; titer by ELISA, 1:60,000) and LPS from the Re mutant of Salmonella minnesota (i.e., to the ketodeoxyoctonate [KDO] and lipid A determinants; titer, 1:3,200). In highly diluted antiserum, titers of antibody to J5 LPS were reduced by 28%-41% after adsorption with seven strains of smooth gram-negative bacteria and by only 4% after adsorption with the Re mutant. Smooth gram-negative bacteria adsorbed virtually all antibody to Re LPS. Therefore, rabbit antiserum to J5 contains type-specific antibodies to core determinants distal to KDO that can obscure highly cross-reactive antibodies to lipid A-KDO in vitro. Cross-reactive antibodies are demonstrable by adsorption with whole bacteria at limiting concentrations of antibody.