Fine mapping and analysis of candidate genes for qBT2 and qBT7.2 locus controlling bolting time in radish (Raphanus sativus L.)
Fine mapping and analysis of candidate genes for qBT2 and qBT7.2 locus controlling bolting time in radish (Raphanus sativus L.)
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DOI：
10.1007/s00122-023-04503-x
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发表时间：
2023-12
影响因子：
5.4
通讯作者：
Yueyue Jin;Xiaobo Luo;Yadong Li;Xiao Peng;Linjun Wu;Guangqian Yang;Xiu-Hong Xu;Yun Pei
中科院分区：
文献类型：
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作者：
Yueyue Jin;Xiaobo Luo;Yadong Li;Xiao Peng;Linjun Wu;Guangqian Yang;Xiu-Hong Xu;Yun Pei
关键信息萝卜抽薹时间的两个主效QTL分别位于第02和07染色体上，分别位于0.37 Mb和0.37 Mb。RsFLC 1和RsFLC 2是萝卜（RaphanussativusL.）是十字花科重要蔬菜作物。萝卜过早抽薹开花会降低肉质根的产量和品质。然而，萝卜抽薹和开花的分子机制仍不清楚。在YZH（早抽薹）× XHT（晚抽薹）F2群体中构建了一张高密度遗传连锁图，遗传距离为2497.74 cM，平均遗传距离为2.31 cM。共检测到9个影响抽薹时间的QTL和2个影响开花时间的QTL。以萝卜GDE（早抽薹）× GDL（晚抽薹）F2群体为材料，利用QTL-seq技术检测到3个与萝卜抽薹时间相关的QTL。精细定位将qBT 2和qBT7.2分别缩小到02和07染色体上的0.37 Mb和0.52 Mb区域。RNA-seq和qRT-PCR分析表明，RsFLC 1和RsFLC 2分别为qBT 7. 2和qBT 2位点的候选基因。亚细胞定位显示RsFLC 1和RsFLC 2主要表达于细胞核。在RsFLC 1的第一个内含子中插入1856-bp的片段，决定了抽薹时间。RsFLC 2的过表达显著延迟了拟南芥的开花。这些研究结果将为探讨萝卜晚抽薹的分子机制提供新的思路，并为萝卜晚抽薹品种的分子标记辅助选择提供参考。
Key messageTwo major QTLs for bolting time in radish were mapped to chromosome 02 and 07 in a 0.37 Mb and 0. 52 Mb interval,RsFLC1andRsFLC2is the critical genes.AbstractRadish (RaphanussativusL.) is an important vegetable crop of Cruciferae. The premature bolting and flowering reduces the yield and quality of the fleshy root of radish. However, the molecular mechanism underlying bolting and flowering in radish remains unknown. In YZH (early bolting) × XHT (late bolting) F2population, a high-density genetic linkage map was constructed with genetic distance of 2497.74 cM and an average interval of 2.31 cM. A total of nine QTLs for bolting time and two QTLs for flowering time were detected. Three QTLs associated with bolting time in radish were identified by QTL-seq using radish GDE (early bolting) × GDL (late bolting) F2population. Fine mapping narrowed downqBT2andqBT7.2to an 0.37 Mb and 0.52 Mb region on chromosome 02 and 07, respectively. RNA-seq and qRT-PCR analysis showed thatRsFLC1andRsFLC2were the candidate gene forqBT7.2andqBT2locus, respectively. Subcellular localization exhibited thatRsFLC1andRsFLC2were mainly expressed in the nucleus. A 1856-bp insertion in the first intron ofRsFLC1was responsible for bolting time. Overexpression ofRsFLC2in Arabidopsis was significantly delayed flowering. These findings will provide new insights into the exploring the molecular mechanism of late bolting and promote the marker-assisted selection for breeding late-bolting varieties in radish.