Arsenate reduction and methylation in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 investigated with X-ray absorption near edge structure.
Arsenate reduction and methylation in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 investigated with X-ray absorption near edge structure.
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DOI：
10.1016/j.jhazmat.2012.09.061
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发表时间：
2012-12
影响因子：
13.6
通讯作者：
S. Su;X. Zeng;L. Li;R. Duan;L. Bai;A. Li;J. Wang;S. Jiang
中科院分区：
文献类型：
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作者：
S. Su;X. Zeng;L. Li;R. Duan;L. Bai;A. Li;J. Wang;S. Jiang
采用同步辐射X射线吸收近边结构(XANES)技术，对具有砷积累和挥发能力的木霉SM-12F1、简青霉SM-12F4和尖孢镰刀菌CZ-8F1细胞中砷的化学形态进行了直接分析。经500mgL−-1处理15d后，Asperellum SM-12F1和P.jantenellum SM-12F4细胞中积累的As(Ⅲ)分别为60.5%和65.3%，其次分别为31.3%和32.4%，8.3%和2.3%。而尖孢镰刀菌CZ-8F1中，As(III)占54.5%，MMA占37.8%，As(V)占7.7%。As(V)的还原和甲基化形成As(III)、MMA和DMA作为主要产物，As(V)的还原可能比甲基化更容易进行。这些结果将有助于理解砷的解毒机理及其在生物修复中的应用前景。
Synchrotron radiation-based X-ray absorption near edge structure (XANES) was introduced to directly analysis chemical species of arsenic (As) in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 capable of As accumulation and volatilisation. After exposure to As(V) of 500mgL−1for 15 days, a total of 60.5% and 65.3% of the accumulated As in the cells of T. asperellum SM-12F1 and P. janthinellum SM-12F4, respectively, was As(III), followed by 31.3% and 32.4% DMA (dimethylarsinic acid), 8.3% and 2.3% MMA (monomethylarsonic acid), respectively. However, for F. oxysporum CZ-8F1, 54.5% of the accumulated As was As(III), followed by 37.8% MMA and 7.7% As(V). The reduction and methylation of As(V) formed As(III), MMA, and DMA as the primacy products, and the reduction of As(V) might be more easily processed than the methylation. These results will help to understanding the mechanisms of As detoxification and its future application in bioremediation.