In vivo evaluation of battery-operated light-emitting diode-based photodynamic therapy efficacy using tumor volume and biomarker expression as endpoints
In vivo evaluation of battery-operated light-emitting diode-based photodynamic therapy efficacy using tumor volume and biomarker expression as endpoints
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DOI：
10.1117/1.jbo.20.4.048003
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发表时间：
2015-04-01
影响因子：
3.5
通讯作者：
Hasan, Tayyaba
中科院分区：
文献类型：
--
作者：
Mallidi, Srivalleesha;Mai, Zhiming;Hasan, Tayyaba
鉴于中低收入国家（LMIC）癌症相关死亡率的上升，迫切需要开发可在最低基础设施机构中使用的经济疗法。光动力疗法（PDT）是一种基于光化学的治疗方式，只要有低成本光源和光敏剂，就提供了这种可能性。在这项原理验证研究中，我们重点关注使 PDT 光源适应低资源环境，并将廉价、便携式、电池供电的发光二极管 (LED) 光源与标准的高成本激光源进行比较。比较研究是在接受 5-氨基乙酰丙酸诱导的原卟啉 IX PDT 的人鳞状细胞癌异种移植小鼠模型中进行的。我们观察到 LED 光源和标准激光光源对肿瘤负荷的控制几乎相同。通过对对照组、LED-PDT 和激光 PDT 治疗组小鼠的坏死、微血管密度和缺氧 [碳酸酐酶 IX (CAIX) 表达] 的生物标志物分析来进一步了解生物反应。用两种不同光源治疗的肿瘤中坏死体积百分比和 CAIX 表达没有显着差异。这些令人鼓舞的初步结果值得在中低收入国家流行的癌症原位动物模型中进行进一步研究。 (C) 作者。由 SPIE 根据 Creative Commons Attribution 3.0 Unported 许可证发布。
In view of the increase in cancer-related mortality rates in low-to middle-income countries (LMIC), there is an urgent need to develop economical therapies that can be utilized at minimal infrastructure institutions. Photodynamic therapy (PDT), a photochemistry-based treatment modality, offers such a possibility provided that low-cost light sources and photosensitizers are available. In this proof-of-principle study, we focus on adapting the PDT light source to a low-resource setting and compare an inexpensive, portable, battery-powered light-emitting diode (LED) light source with a standard, high-cost laser source. The comparison studies were performed in vivo in a xenograft murine model of human squamous cell carcinoma subjected to 5-aminolevulinic acid-induced protoporphyrin IX PDT. We observed virtually identical control of the tumor burden by both the LED source and the standard laser source. Further insights into the biological response were evaluated by biomarker analysis of necrosis, microvessel density, and hypoxia [carbonic anhydrase IX (CAIX) expression] among groups of control, LED-PDT, and laser-PDT treated mice. There is no significant difference in the percent necrotic volume and CAIX expression in tumors that were treated with the two different light sources. These encouraging preliminary results merit further investigations in orthotopic animal models of cancers prevalent in LMICs. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.