Expression of ammonia transporter family members, Rh B glycoprotein and Rh C glycoprotein, in the developing rat kidney.
Expression of ammonia transporter family members, Rh B glycoprotein and Rh C glycoprotein, in the developing rat kidney.
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氨转运蛋白家族成员 Rh B 糖蛋白和 Rh C 糖蛋白在发育中的大鼠肾脏中的表达。
DOI：
10.1152/ajprenal.00607.2009
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Weiner,IDavid
中科院分区：
文献类型：
--
作者：
Han,Ki-Hwan;Lee,Su-Youn;Kim,Wan-Young;Shin,Jung-A;Kim,Jin;Weiner,IDavid
氨代谢是酸碱平衡的主要组成部分，但在出生时发育不完全。Rh B糖蛋白（Rhbg）和Rh C糖蛋白（Rhcg）是新近发现的表达于哺乳动物肾脏的氨转运蛋白家族成员。本研究的目的是确定Rhbg和Rhcg在肾脏发育过程中的表达和定位。我们检查了胎儿16天（E16）、18天（E18）和20天（E20）以及出生后发育的前21天的肾脏。Rhbg最初在E18表达，仅在连接小管（CNT）中表达;在E20，Rhbg在CNT和髓集合管（MCD）中表达。相反，Rhcg首先在E16表达，在输尿管芽中表达为基底表达;在E18，其在CNT细胞亚群中表达为顶端模式，随后在E20在MCD中表达为顶端和基底侧表达。在皮质中，Rhbg和Rhcg表达增加的CNT表达之前，在胎儿发育过程中的皮质集合管。在MCD中，Rhbg和Rhcg表达最初在乳头状尖端的细胞中，在胎儿晚期逐渐从尖端去除，并在新生儿早期过渡到成人模式，主要表达在外部MCD中，仅在初始内部MCD的细胞中罕见表达。用嵌入细胞特异性标记物进行双标记，发现Rhbg和Rhcg最初在CNT细胞、CNT A型嵌入细胞和非A、非B嵌入细胞以及MCD A型嵌入细胞中表达。我们得出结论，Rhbg和Rhcg的表达平行于闰细胞的发育，出生时未成熟的Rhbg和Rhcg表达有助于不完全的氨排泄能力。
Ammonia metabolism is a primary component of acid-base homeostasis but is incompletely developed at time of birth. Rh B glycoprotein (Rhbg) and Rh C glycoprotein (Rhcg) are recently recognized ammonia transporter family members expressed in the mammalian kidney. This study's purpose was to establish the expression and localization of Rhbg and Rhcg during kidney development. We examined kidneys from fetaldays 16(E16),18(E18), and20(E20), and from the first 21 days of postnatal development. Rhbg was expressed initially atE18, with expression only in the connecting tubule (CNT); atE20, Rhbg was expressed in both the CNT and the medullary collecting duct (MCD). In contrast, Rhcg was first expressed atE16with basal expression in the ureteric bud; atE18, it was expressed in a subset of CNT cells with an apical pattern, followed by apical and basolateral expression in the MCD atE20. In the cortex, Rhbg and Rhcg expression increased in the CNT before expression in the cortical collecting duct during fetal development. In the MCD, both Rhbg and Rhcg expression was initially in cells in the papillary tip, with gradual removal from the tip during the late fetal period and transition during the early neonatal period to an adult pattern with predominant expression in the outer MCD and only rare expression in cells in the initial inner MCD. Double-labeling with intercalated cell-specific markers identified that Rhbg and Rhcg were expressed initially in CNT cells, CNT A-type intercalated cells and non-A, non-B intercalated cells, and in MCD A-type intercalated cells. We conclude that expression of Rhbg and Rhcg parallels intercalated cell development and that immature Rhbg and Rhcg expression at birth contributes to incomplete ammonia excretion capacity.