Hair shaft elongation, follicle growth, and spontaneous regression in long-term, gelatin sponge-supported histoculture of human scalp skin.
Hair shaft elongation, follicle growth, and spontaneous regression in long-term, gelatin sponge-supported histoculture of human scalp skin.
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DOI：
10.1073/pnas.89.18.8764
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发表时间：
1992-09
影响因子：
11.1
通讯作者：
L. Li;L. Margolis;R. Paus;R. Hoffman
中科院分区：
文献类型：
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作者：
L. Li;L. Margolis;R. Paus;R. Hoffman
为了更好地了解人类毛发生长控制的分子机制和测试毛发生长调节药物，需要合适的体外模型。在这里，我们报告了在完整头皮组织培养中人类毛囊的长期生长、毛干伸长和自发退化。在空气/液体界面的明胶海绵支持的组织培养系统中，在毛发生长周期的各个阶段具有丰富毛囊的人头皮皮肤生长长达40天。孤立的毛囊放置在明胶海绵基质中也支持毛干伸长，毛囊细胞保持增殖和存活很长一段时间。毛干的延长主要发生在完整皮肤和孤立毛囊组织培养的前10天。然而，毛囊是可行的，毛囊角质形成细胞继续纳入[3 H]胸苷长达数周后，轴伸长已停止所示的荧光染料双染色，测量共聚焦激光扫描显微镜，并通过组织学放射自显影[3 H]胸苷掺入，分别。毛囊可以在组织培养中继续其周期;例如，在组织学上和毛囊的实际退化中都观察到明显的自发性退行期诱导。此外，在培养开始后第40天，显示了毫毛卵泡是存活的。在组织培养的人头皮中，我们证明了肥大细胞与生长期毛囊和巨噬细胞与退化期毛囊的关系，表明这些细胞在头发周期中的作用。这种组织培养技术应该作为一个强大的工具，为未来的头发研究在人类系统，以及筛选分析的化合物，可以扰乱头发周期。
In order to better understand the molecular mechanisms of human hair growth control and to test hair growth-modulatory drugs, appropriate in vitro models are required. Here, we report the long-term growth, shaft elongation, and spontaneous regression of human hair follicles in histoculture of intact scalp skin. Human scalp skin with abundant hair follicles in various stages of the hair growth cycle was grown for up to 40 days in a gelatin sponge-supported histoculture system at the air/liquid interface. Isolated follicles placed in the gelatin-sponge matrix also supported hair shaft elongation, with the hair follicle cells remaining proliferative and viable for very long periods. Hair shaft elongation occurred mainly during the first 10 days of histoculture of both intact skin and isolated follicles. However, hair follicles were viable and follicle keratinocytes continued to incorporate [3H]thymidine for up to several weeks after shaft elongation had ceased as shown by fluorescent-dye double staining, measured by confocal laser scanning microscopy, and by histological autoradiography of [3H]thymidine incorporation, respectively. Hair follicles could continue their cycle in histoculture; for example, apparent spontaneous catagen induction was observed both histologically and by the actual regression of the hair follicle. In addition, vellus follicles were shown to be viable at day 40 after initiation of culture. In the histocultured human scalp we demonstrated the association of mast cells with anagen follicles and macrophages with catagen follicles, suggesting a role of these cells in the hair cycle. This histoculture technique should serve as a powerful tool for future hair research in the human system as well as a screening assay for compounds that can perturb the hair cycle.