A Ratiometric Electrochemical Sensor with Integrated Probe for the Assay of alpha-glucosidase Activity and Screening of Its Inhibitors
A Ratiometric Electrochemical Sensor with Integrated Probe for the Assay of alpha-glucosidase Activity and Screening of Its Inhibitors
复制标题
带有集成探针的比率电化学传感器用于测定α-葡萄糖苷酶活性及其抑制剂的筛选
DOI：
10.1149/2.1111902jes
复制
发表时间：
2019
影响因子：
3.9
通讯作者：
Zhao Peng
中科院分区：
文献类型：
--
作者：
Liu Yanyan;Li Hongmei;Lu Ling;Sun Bin;Huang Lihui;Chen Hongyu;Qiu Wenhua;Tao Jia;Zhao Peng
本文采用由两个氧化还原基团而不是两个氧化还原分子组成的集成电化学探针，该集成探针同时提供参考信号和目标响应信号。结合纳米碳角修饰电极增大电活性表面积和促进电子转移的优点，制备了一种用于α-葡萄糖苷酶及其抑制剂检测的新型比例电化学传感器。α-葡萄糖苷酶的检测范围为0.001 ~ 0.016 U/mL，检出限为0.00056 U/mL，低于大多数现有方法。以阿卡波糖和桑皮两种α-葡萄糖苷酶抑制剂作为筛选潜在降糖药物的可行性评估，其IC 50值分别为0.55 mM和3.38 mg/mL。因此，我们提出的传感策略为新型比例电化学传感器的设计开辟了道路，并极大地扩展了电化学在医学分析中的应用。
In this paper, we utilized an integrated electrochemical probe which consist of two redox groups instead of two redox molecules, the integrated probe simultaneously provided referenced signal and target responded signal. Combining with merits of enlarging the electroactive surface area and promoting the electron transfer of carbon nanohorns modified electrode, a new ratiometric electrochemical sensor for the assay of α-glucosidase and its inhibitors was fabricated. The ratiometric signal enabled the detection of α-glucosidase in a range of 0.001–0.016 U/mL with a detection limit of 0.00056 U/mL, which is lower than most of the existing methods. Furthermore, two α-glucosidase inhibitors, acarbose and mulberry bark, were used to assess the feasibility of screening potential anti-diabetic medicine, and the IC 50 values of 0.55 mM and 3.38 mg/mL were obtained respectively. Thus, our proposed sensing strategy broadens the way for the design of novel ratiometric electrochemical sensors, and greatly extended the application of electrochemistry in medical analysis.