Phase diversity for three-dimensional imaging
Phase diversity for three-dimensional imaging
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DOI：
10.1364/josaa.30.001980
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发表时间：
2013-10-01
影响因子：
1.9
通讯作者：
Kner, Peter
中科院分区：
文献类型：
--
作者：
Kner, Peter
相位分集（PD）是一种从扩展场景的二维图像中估计波前像差的强大技术。PD可以与扩展的非相干图像一起工作，并且在自适应光学系统中，除了可变形反射镜之外不需要额外的硬件。由于这些原因，PD应该非常适合于显微镜应用中的像差测量。但是，在生物宽视场显微镜中，被成像的物体通常是三维的，并且图像包含散焦光。在本文中，我们介绍了多平面PD和PD可以扩展到三维物体的宽场成像。这在生物荧光显微镜领域应该是特别有用的，在该领域中，对象是非常光敏感的，并且像差不能容易地被确定。(C)2013年美国光学学会
Phase diversity (PD) is a powerful technique for estimating wavefront aberrations from two-dimensional images of extended scenes. PD can work with extended incoherent images and, in an adaptive optics system, does not need extra hardware in addition to the deformable mirror. For these reasons, PD should be well suited to aberration measurement in microscopy applications. But, in biological widefield microscopy, the objects being imaged are frequently three-dimensional, and the images contain out-of-focus light. In this paper, we introduce multiplane PD and show that PD can be extended to widefield imaging of three-dimensional objects. This should be particularly useful in the field of biological fluorescence microscopy where the objects are very light sensitive and the aberrations cannot easily be determined. (C) 2013 Optical Society of America