Quantification of cell adhesion using a spinning disc device and application to surface-reactive materials
Quantification of cell adhesion using a spinning disc device and application to surface-reactive materials
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DOI：
10.1016/s0142-9612(97)00042-2
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发表时间：
1997-08-01
期刊：
影响因子：
14
通讯作者：
Boettiger, D
中科院分区：
文献类型：
--
作者：
Garcia, AJ;Ducheyne, P;Boettiger, D
细胞黏附的定量分析对于理解生理现象和开发生物技术应用是必不可少的。电化学测量表明，与旋转圆盘装置相关的输运模式接近于圆盘在无限大流体中旋转时的流体流动和传质场。因此，该装置在界面上均匀且恒定的化学条件下，对附着的细胞施加线性范围的力。通过研究成骨样细胞与纤维连接蛋白在不同化学环境下吸附在生物活性和非反应性玻璃上的粘附性，说明了该仪器在检测细胞与表面活性物质粘附性方面的应用。将细胞接种于纤维连接蛋白包被的基质上15min，然后在分离力作用下10min。贴壁细胞的数量随着施加的力呈非线性减少，脱离轮廓可通过S型曲线拟合准确描述，如预期的那样，具有正态分布的黏附属性的细胞群体。(C)1997年爱思唯尔科学有限公司。版权所有。
Quantitative analysis of cell adhesion is essential in understanding physiological phenomena and developing biotechnological applications. Electrochemical measurements demonstrated that the transport patterns associated with a spinning disc device approximate the fluid flow and mass transport fields for a disc spinning in an infinite fluid. Therefore, this device applies a linear range of forces to attached cells under uniform and constant chemical conditions at the interface. The application of this apparatus for examining cell adhesion to surface-active materials was illustrated by investigating the attachment of osteoblast-like cells to fibronectin adsorbed onto bioactive and nonreactive glasses for different chemical environments. Cells were seeded on fibronectin-coated substrates for 15 min and then subjected to detachment forces for 10 min. The number of adherent cells decreased non-linearly with applied force and the detachment profile was accurately described by a sigmoidal curve fit, as expected for a cell population with normally distributed adhesion properties. (C) 1997 Elsevier Science Limited. All rights reserved.