Treatment of partial growth arrest using an in vitro-generated scaffold-free tissue-engineered construct derived from rabbit synovial mesenchymal stem cells
Treatment of partial growth arrest using an in vitro-generated scaffold-free tissue-engineered construct derived from rabbit synovial mesenchymal stem cells
复制标题
使用源自兔滑膜间充质干细胞的体外产生的无支架组织工程构建体治疗部分生长停滞
DOI：
10.1097/bpo.0b013e31824afee3
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Yoshikawa H
中科院分区：
文献类型：
--
作者：
Yoshida K;Higuchi C;Nakura A;Nakamura N;Yoshikawa H
背景：骺板损伤有时会导致部分生长停滞，这可能导致生长期儿童出现角畸形和肢体长度差异。本研究的目的是开发一种新的治疗部分生长停滞的生长骨骺板。为了这个目的，我们调查的可行性，在体外生成的无支架组织工程化结构（TEC）来自滑膜间充质干细胞（MSCs）在兔生长停滞model.Methods：生长停滞的实验模型是通过切除内侧一半的近端生长板的胫骨从6周龄的新西兰白色兔。结果：TEC植入组4周后，生长板全部停止生长，4周后出现成角畸形。在第2组和第3组中，角变形小于对照组。组织学图像显示第1组在受损的生长板处形成骨桥。在第2组和第3组中确认生长板再生。组织学检查显示，组3中的生长板比组2中的生长板再生更大。结论：利用培养的滑膜来源的间充质干细胞制备的无支架三维软骨细胞可分化为增殖性和前肥大性软骨细胞样细胞。临床意义：本实验研究的结果表明，利用培养的滑膜来源的间充质干细胞制备的无支架三维软骨细胞可为骨骺损伤的修复提供一种新的途径。无支架3D TEC用于生长停滞的临床有效性仍有待确定。
Background:Injuries to the epiphyseal plate sometimes result in partial growth arrest, which can lead to the development of angular deformities and limb length discrepancies in growing children. The aim of this study was to develop a new treatment for partial growth arrest of the physis. For this purpose, we investigated the feasibility of an in vitro-generated scaffold-free tissue-engineered construct (TEC) derived from synovial mesenchymal stem cells (MSCs) in a rabbit growth arrest model.Methods:An experimental model for growth arrest was created by excising the medial half of the proximal growth plate of tibias from 6-week-old New Zealand White rabbits. Three experimental groups were set to evaluate TEC implantation: group 1, no implantation as controls; group 2, implantation of bone wax as additional controls; and group 3, implantation of TEC in the lesion.Results:In group 1, all damaged growth plates were arrested and angular deformities appeared 4 weeks later. In groups 2 and 3, angular deformities were less than in the control group. Histologic images showed bone bridges developed at the damaged growth plate in group 1. Regeneration of growth plates was recognized in groups 2 and 3. Histologic examination showed greater regeneration of the growth plate in group 3 than in group 2. In addition, MSCs in the TEC differentiated into proliferative and prehypertrophic chondrocyte-like cells.Conclusions:A scaffold-free 3D TEC made using cultured synovium-derived MSCs differentiated into proliferative and prehypertrophic chondrocyte-like cells.Clinical Relevance:The results of this experimental study suggest that scaffold-free 3D TEC made using cultured synovium-derived MSCs can be a new approach for the repair of epiphyseal injury. Clinical effectiveness of a scaffold-free 3D TEC for growth arrest remains to be determined.