CHANGE IN SOLUBILITY OF TYPE-I COLLAGEN IN HUMAN UTERINE CERVIX IN PREGNANCY AT TERM
CHANGE IN SOLUBILITY OF TYPE-I COLLAGEN IN HUMAN UTERINE CERVIX IN PREGNANCY AT TERM
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DOI：
10.1016/0006-2944(79)90081-4
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
HIRAKAWA, S
中科院分区：
文献类型：
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作者：
ITO, A;KITAMURA, K;HIRAKAWA, S
本文研究了足月妊娠人宫颈胶原蛋白的变化。每干组织的胶原蛋白浓度显著降低至.apprx。50%的对照组和90%以上的宫颈胶原是不溶的。用胃酶消化法提取不溶性胶原蛋白，APPRX。对照组52%的不溶性胶原蛋白和足月妊娠时94%的不溶性胶原蛋白被溶解。用不同的盐析方法分离得到不同遗传差异的I型和III型胶原蛋白，并通过离子交换层析、十二烷基硫酸钠圆盘凝胶电泳法和氨基酸组成分析对其进行鉴定。尽管正常对照组和足月妊娠组胃酶可溶胶原组分的比例不同，但组分中的I/III型比率分别约为2和4.7。溴化氰胶原肽分析对照的胃酶不溶性残留物为.apprx。I/III型胶原占足月全胶原的比例接近4.7，说明I型胶原的溶解度随妊娠时间的延长而显著增加。胶原浓度的降低和I型胶原溶解度的增加是导致足月妊娠宫颈扩张消退的重要因素。
Collagen changes in the human uterine cervix in pregnancy at term were investigated. Collagen concentration per dry tissue significantly decreased to .apprx. 50% of the control and more than 90% of cervical collagen was insoluble. The insoluble collagen was extracted by pepsin digestion, and .apprx. 52% of insoluble collagen in the control and 94% at pregnancy at term were solubilized. In pepsin-soluble fractions, genetically distinct types I and III collagens were isolated by different salt precipitation and identified by ion-exchange chromatography, SDS disc gel electrophoresis and amino acid composition analysis. Although the rates of pepsin-soluble collagen fraction in control and in pregnancy at term were different, types I/III ratios in the fraction were about 2 and 4.7, respectively. The cyanogen bromide collagen peptide analyses of pepsin-insoluble residues of control which was .apprx. 50% of whole collagen indicated that most of residual collagen was type I. The ratio of types I/III collagen in control of whole collagen approaches the value of 4.7 in pregnancy at term, indicating that the solubility of type I collagen was significantly increased by pregnancy. Both decrease in collagen concentration and increase in type I collagen solubility are important factors which induce the cervical dilatation and effacement in pregnancy at term.