Production of bioactive human granulocyte-colony stimulating factor in transgenic rice cell suspension cultures
Production of bioactive human granulocyte-colony stimulating factor in transgenic rice cell suspension cultures
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DOI：
10.1016/j.pep.2005.09.028
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发表时间：
2006-05-01
影响因子：
1.6
通讯作者：
Yang, Moon-Sik
中科院分区：
文献类型：
--
作者：
Hong, Shin-Young;Kwon, Tae-Ho;Yang, Moon-Sik
在转基因水稻细胞悬浮培养中表达了人粒细胞集落刺激因子（hG-CSF）。将g - csf基因克隆到含有水稻α -淀粉酶基因Amy3D启动子、信号肽和终止子的水稻表达载体中。利用颗粒轰击介导转化技术，将hG-CSF基因导入水稻东晋愈伤组织。ELISA和Northern blot分析证实了hG-CSF基因的表达。采用时间序列ELISA法测定糖饥饿条件下转基因水稻细胞悬浮培养培养基中重组hG-CSF的积累量。通过测量AML-193细胞的增殖证实了植物源性hG-CSF的生物活性，并且与商业大肠杆菌源性hG-CSF的生物活性相似。在本文中，我们讨论了利用水稻细胞悬浮系统表达治疗性重组hG-CSF的吸引力。(c) 2005爱思唯尔公司版权所有。
Human granulocyte-colony stimulating factor (hG-CSF), a human cytokine, was expressed in transgenic rice cell suspension culture. The hG-CSF gene was cloned into the rice expression vector containing the promoter, signal peptide, and terminator derived from a rice alpha-amylase gene Amy3D. Using particle bombardment-mediated transformation, hG-CSF gene was introduced into the calli of rice (Oryza sativa) cultivar Dong-jin. Expression of the hG-CSF gene was confirmed by ELISA and Northern blot analysis. The amount of recombinant hG-CSF accumulated in culture medium from transgenic rice cell suspension culture on the sugar starvation was determined by time series ELISA. Biological activity of the plant derived hG-CSF was confirmed by measuring the proliferation of the AML-193 cells, and was similar to that of the commercial Escherichia coli-derived hG-CSF. In this paper, we discuss the attractive attributes of using rice cell suspension system for the expression of therapeutic recombinant hG-CSF. (c) 2005 Elsevier Inc. All rights reserved.