Expression of aquaporin water channels in the rat pituitary gland
Expression of aquaporin water channels in the rat pituitary gland
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DOI：
10.1292/jvms.69.1175
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发表时间：
2007-11-01
影响因子：
1.2
通讯作者：
Seki, Makoto
中科院分区：
文献类型：
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作者：
Kuwahara, Sachi;Maeda, Seisbi;Seki, Makoto
我们通过逆转录-聚合酶链反应（RT-PCR）和免疫组织化学方法研究了水通道蛋白（AQP）亚型（AQP 1-AQP 9）在大鼠脑垂体中的表达。RT-PCR检测结果显示，AQP 1、AQP 3、AQP 4和AQP 5有特异性扩增，而其它AQP 5无特异性扩增。血管内皮细胞中检测到AQP 1免疫反应。免疫组化检测AQP 3 mRNA表达，未见AQP 3阳性反应。AQP 4定位于腺垂体和神经垂体的支持细胞。AQP 5在Rathke残余囊的部分边缘细胞表达。这些观察结果表明，AQP家族的几个亚型参与调节大鼠脑垂体的水稳态。
We investigated the expression of aquaporin (AQP) subtypes (AQP1-AQP9) in the rat pituitary gland by the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Specific amplification by RT-PCR was observed in AQP1, AQP3, AQP4 and AQP5 but not in the others. AQP1 immunoreactivity was detected in the endothelial cells of blood vessels. No positive reaction to AQP3 was observed by immunohistochemistry, in spite of the detection of mRNA. AQP4 was localized in the supporting cells of the adenohypophysis and neurohypophysis. AQP5 was expressed in parts of the marginal cells in Rathke's residual pouch. These observations suggest that several subtypes of the AQP family are involved in regulation of water homeostasis in the rat pituitary gland.