Dopaminergic innervation of the rat globus pallidus characterized by microdialysis and immunohistochemistry
Dopaminergic innervation of the rat globus pallidus characterized by microdialysis and immunohistochemistry
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DOI：
10.1007/s00221-003-1638-7
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发表时间：
2004-01-01
影响因子：
2
通讯作者：
Hauber, W
中科院分区：
文献类型：
--
作者：
Fuchs, H;Hauber, W
本研究通过体内微透析和免疫组织化学更详细地检查了大鼠苍白球的多巴胺能神经支配。使用酪氨酸羟化酶免疫组织化学，在苍白球中从形态上区分了两类多巴胺能纤维。将 6-羟基多巴胺单侧输注到黑质中会导致苍白球中多巴胺能纤维密度的损失，这与病变的黑质范围相关。这些发现与黑质多巴胺能细胞体的退行性丧失也可能影响纹状体外结构（如苍白球）的多巴胺输入的观点一致。使用体内微透析，我们测试了苍白球中测量的多巴胺是否是神经元来源的。河豚毒素的灌注引起苍白球多巴胺的强烈且短暂的减少。苍白球多巴胺的河豚毒素敏感性证明了黑质苍白球多巴胺能神经支配的功能意义。
The present study examined the dopaminergic innervation of the rat globus pallidus by in vivo microdialysis and immunohistochemistry in more detail. Using tyrosine hydroxylase immunohistochemistry, two classes of dopaminergic fibers were distinguished morphologically in the globus pallidus. Unilateral infusion of 6-hydroxydopamine into the substantia nigra produced a loss of dopaminergic fiber density in the globus pallidus which was correlated with the nigral extent of the lesion. These findings are in line with the notion that a degenerative loss of nigral dopaminergic cell bodies might also affect the dopamine input of extrastriatal structures such as the globus pallidus. Using in vivo microdialysis, we tested whether dopamine measured in the globus pallidus is of neuronal origin. Perfusion of tetrodotoxin induced a strong and transient decrease of pallidal dopamine. The tetrodotoxin-sensitivity of pallidal dopamine demonstrates the functional significance of the nigropallidal dopaminergic innervation.