Characterization and expression of an nsLTPs-like antimicrobial protein gene from motherwort (Leonurus japonicus)
Characterization and expression of an nsLTPs-like antimicrobial protein gene from motherwort (Leonurus japonicus)
复制标题
益母草 (益母草) nsLTPs 样抗菌蛋白基因的表征和表达
DOI：
10.1007/s00299-008-0506-0
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发表时间：
2008-04-01
影响因子：
6.2
通讯作者：
Pei, Yan
中科院分区：
文献类型：
--
作者：
Yang, Xingyong;Wang, Xiaowen;Pei, Yan
为了从植物种子中筛选有效的抗菌蛋白（AMP），我们从中药益母草（Leonurus japonicusHoutt）的种子中纯化了一种热稳定的AMP，LJAMP 2。在体外试验中，该蛋白质可以抑制真菌和细菌的生长。根据测定的N端氨基酸序列，通过快速扩增cDNA末端，克隆了编码LJAMP2的cDNA。推导的氨基酸序列与植物非特异性脂转运蛋白相似。北方印迹分析表明，该基因在种子中表达，命名为LJAMP 2。在烟草中过量表达LJAMP 2可显著提高烟草对真菌病原菌Alternaria alternata和细菌病原菌Ralstoniasolanacearum的抗性，而对植株的生长发育无明显影响。我们的数据证实了益母草种子中LJAMP 2的抗真菌和抗细菌功能，并表明LJAMP 2在提高植物抗病性方面的潜力。
In screening for potent antimicrobial proteins (AMPs) from plant seeds, we had purified a heat-stable AMP, LJAMP2, from the seeds of a medicine herb, motherwort (Leonurus japonicusHoutt). In an in vitro assay, the protein can inhibit the growth of both fungi and bacteria. Then a cDNA encoding LJAMP2 was cloned by the rapid amplification of cDNA ends based on the N-terminal amino acid sequence determined. The deduced amino acid sequences of this cDNA show similarity to plant non-specific lipid transfer proteins. Northern blotting assay revealed that this nsLTP-like gene, designatedLJAMP2, was expressed in seeds. Overexpression ofLJAMP2in tobacco enhanced resistance to the fungal pathogenAlternaria alternataand the bacterial pathogenRalstoniasolanacearum, significantly, while no visible alteration in plant growth and development. Our data confirm the antifungal and antibacterial function ofLJAMP2from motherwort seeds and suggest the potential ofLJAMP2in improving disease resistance in plants.