Quorum sensing controls Vibrio cholerae multicellular aggregate formation
Quorum sensing controls Vibrio cholerae multicellular aggregate formation
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DOI：
10.7554/elife.42057
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发表时间：
2018-12-24
期刊：
影响因子：
7.7
通讯作者：
Bassler, Bonnie L.
中科院分区：
文献类型：
--
作者：
Jemielita, Matthew;Wingreen, Ned S.;Bassler, Bonnie L.
细菌使用一种称为群体感应（QS）的过程进行交流并共同调节基因表达。QS依赖于对称为自身诱导物的信号分子的群体范围的反应。在这里，我们表明，QS激活霍乱弧菌的多细胞性的新程序。这个程序，我们称之为聚集，是不同于典型的表面生物膜形成程序，其中QS抑制。聚集由自身诱导物诱导，在细胞悬浮液中快速发生，并且不需要细胞分裂，其特征与霍乱弧菌生物膜形成的特征显著不同。细胞外DNA限制聚集体大小，但不足以驱动聚集。诱变筛选鉴定了聚集体形成所需的基因，揭示了参与霍乱弧菌肠道定殖、应激反应的蛋白质，以及将当前霍乱弧菌大流行菌株与早期大流行菌株区分开的蛋白质。我们认为，QS控制的聚集体的形成是很重要的霍乱弧菌之间的海洋生态位和人类宿主成功过境。
Bacteria communicate and collectively regulate gene expression using a process called quorum sensing (QS). QS relies on group-wide responses to signal molecules called autoinducers. Here, we show that QS activates a new program of multicellularity in Vibrio cholerae. This program, which we term aggregation, is distinct from the canonical surface-biofilm formation program, which QS represses. Aggregation is induced by autoinducers, occurs rapidly in cell suspensions, and does not require cell division, features strikingly dissimilar from those characteristic of V. cholerae biofilm formation. Extracellular DNA limits aggregate size, but is not sufficient to drive aggregation. A mutagenesis screen identifies genes required for aggregate formation, revealing proteins involved in V. cholerae intestinal colonization, stress response, and a protein that distinguishes the current V. cholerae pandemic strain from earlier pandemic strains. We suggest that QS-controlled aggregate formation is important for V. cholerae to successfully transit between the marine niche and the human host.