Hyaluronidase inhibitory saponins and a trypanocidal isoflavonoid from the aerial parts of Oxytropis lanata
Hyaluronidase inhibitory saponins and a trypanocidal isoflavonoid from the aerial parts of Oxytropis lanata
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DOI：
10.1016/j.fitote.2020.104608
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发表时间：
2020-09-01
期刊：
影响因子：
3.4
通讯作者：
Sasaki, Kenroh
中科院分区：
文献类型：
--
作者：
Buyankhishig, Buyanmandakh;Murata, Toshihiro;Sasaki, Kenroh
对来自羊毛棘豆地上部分的提取物进行化学检测，分离并鉴定出36种化合物，包括皂苷、甘草酸、恶唑和糖苷。其中3个化合物为未见报道的齐墩果烷型皂苷。在杀锥虫筛选中，5，7，4 '-三羟基异黄酮显示出对刚果锥虫（Trypanosoma congolense）（动物中非洲锥虫病的病原体）的抑制活性（IC 50 = 10.5 μ M）;该活性与来自该植物根的活性化合物的活性相似。O.已知羊毛脂是蒙古用于治疗炎性疾病的传统药用植物。皂苷3、5、8和9的抗透明质酸酶作用（IC 50 = 0.15-0.22 mM）强于用作参比药物的色甘酸钠（IC 50 = 0.37 mM）。新皂苷的化学结构基于HRFABMS、H-1和C-13 NMR、H-1-H-1 COSY、HMQC、HMBC和ROESY光谱数据沿着化学程序来确定。
chemical examination of an extract from the aerial part of Oxytropis lanata led to the isolation and identification of 36 compounds, including saponins, isoflavonoids, oxazoles, and glycosides. The three among them were previously unreported oleanane-type saponins. In trypanocidal screening, 5,7,4'-trihydroxyisoflavone showed inhibitory activity against Trypanosoma congolense (IC50 = 10.5 mu M), the causative agent of African trypanosomosis in animals; this activity was similar to that of active compounds from the roots of this plant. O. lanata is known to be a traditional medicinal plant in Mongolia for the treatment of inflammatory diseases. The anti-hyaluronidase effect of saponins 3, 5, 8, and 9, (IC50 = 0.15-0.22 mM) was stronger than that of sodium cromoglicate, which was used as a reference drug (IC50 = 0.37 mM). The chemical structures of the new saponins were determined based on HRFABMS, H-1 and C-13 NMR, H-1--H-1 COSY, HMQC, HMBC, and ROESY spectroscopic data along with chemical procedures.