INFLUENCE OF THE ENDOGENOUS STORAGE LIPID POLY-BETA-HYDROXYBUTYRATE ON THE REDUCING POWER-AVAILABILITY DURING COMETABOLISM OF TRICHLOROETHYLENE AND NAPHTHALENE BY RESTING METHANOTROPHIC MIXED CULTURES
INFLUENCE OF THE ENDOGENOUS STORAGE LIPID POLY-BETA-HYDROXYBUTYRATE ON THE REDUCING POWER-AVAILABILITY DURING COMETABOLISM OF TRICHLOROETHYLENE AND NAPHTHALENE BY RESTING METHANOTROPHIC MIXED CULTURES
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DOI：
10.1128/aem.59.5.1602-1606.1993
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发表时间：
1993-05-01
影响因子：
4.4
通讯作者：
MCCARTY, PL
中科院分区：
文献类型：
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作者：
HENRYSSON, T;MCCARTY, PL
研究了储存脂质聚 13-羟基丁酸酯 (PHB) 在甲烷氧化混合培养物转化三氯乙烯中的作用。萘氧化率用于测定可溶性甲烷单加氧酶活性。反应器中生长的甲烷氧化细胞的 PHB 含量每天都在变化。细胞内PHB含量与萘氧化速率、三氯乙烯转化率和容量均呈正相关。添加β-羟基丁酸酯显着增加了萘的氧化速率。细胞中的 PHB 含量可以通过在不同的甲烷与氮气比率下孵育来控制。可以看出，在这些孵育后，萘氧化速率与 PHB 含量呈正相关。在暴露于氧气的静息甲烷氧化细胞中，PHB 含量和萘氧化速率都随着时间的推移而降低。然而，萘氧化速率的降低不能仅用 PHB 含量的降低来解释。可能还涉及甲烷单加氧酶本身的失活。
The role of the storage lipid poly-13-hydroxybutyrate (PHB) in trichloroethylene transformation by methanotrophic mixed cultures was investigated. Naphthalene oxidation rates were used to assay for soluble methane monooxygenase activity. The PHB content of methanotrophic cells grown in reactors varied diurnally as well as from day to day. A positive correlation between the amount of PHB in the cells and the naphthalene oxidation rate as well as between PHB and the trichloroethylene transformation rate and capacity was found. Addition of beta-hydroxybutyrate increased the naphthalene oxidation rates significantly. PHB content in cells could be manipulated by incubation at different methane-to-nitrogen ratios. A positive correlation between the naphthalene oxidation rate and the PHB content after these incubations could be seen. Both the PHB content and the naphthalene oxidation rates decreased with time in resting methanotrophic cells exposed to oxygen. However, this decrease in the naphthalene oxidation rate cannot be explained by the decrease in the PHB content alone. Probably a deactivation of the methane monooxygenase itself is also involved.