Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice
Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice
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DOI：
10.1007/s00299-003-0698-2
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发表时间：
2004-02-01
影响因子：
6.2
通讯作者：
Toriyama, K
中科院分区：
文献类型：
--
作者：
Okuzaki, A;Toriyama, K
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Site-specific mutagenesis in a rice genome was obtained by introducing chimeric RNA/DNA oligonucleotides (COs) by means of particle bombardment. Three COs were designed to target the independent codons for Pro-171, Trp-548 and Ser-627 of the endogenous rice acetolactate synthase (ALS) gene so it would confer resistance to ALS-inhibiting herbicides. Sequencing of the ALS gene of herbicide-resistant plants demonstrated that the ALS sequence was modified in a site-specific fashion. The efficiency of gene conversion mediated by COs was estimated to be 1x10(-4). These results demonstrate that CO-directed gene targeting is feasible in rice.