Sustained and dynamic inositol lipid metabolism inside and outside the immunological synapse
Sustained and dynamic inositol lipid metabolism inside and outside the immunological synapse
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DOI：
10.1038/ni848
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发表时间：
2002-11-01
影响因子：
30.5
通讯作者：
Cantrell, DA
中科院分区：
文献类型：
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作者：
Costello, PS;Gallagher, M;Cantrell, DA
T细胞活化是通过与抗原呈递细胞（APCs）表面的肽-主要组织相容性（MHC）复合物接触数小时触发的。T细胞激活所需的持续信号转导途径的性质和位置尚不清楚。我们在这里表明，当T细胞对抗原作出反应时，磷脂酰肌醇（3,4,5）三磷酸（PIP3）的产生可以动态持续数小时。此外，PIP3的持续升高对T细胞增殖至关重要。在T细胞- apc接触区和细胞对跖极有PIP3积累。因此，免疫突触不是激活T细胞中持续信号转导的唯一位点。
T cell activation is triggered by several hours of contact with peptide-major histocompatibility (MHC) complexes on the surface of antigen-presenting cells (APCs). The nature and location of the sustained signal transduction pathways required for T cell activation are unknown. We show here that the production of phosphatidylinositol (3,4,5)triphosphate (PIP3) was dynamically sustained for hours as T cells responded to antigen. In addition, sustained elevation of PIP3 was essential for T cell proliferation. There was PIP3 accumulation in the T cell-APC contact zone and at the antipodal pole of the cell. The immune synapse is thus not the sole site of sustained signal transduction in activated T cells.