Identification of melanoma-associated antigens using fixed tissue screening of antibodies.
Identification of melanoma-associated antigens using fixed tissue screening of antibodies.
复制标题
DOI：
--
复制
发表时间：
1984-06
期刊：
影响因子：
11.2
通讯作者：
B. Atkinson;C. Ernst;B. Ghrist;M. Herlyn;M. Błaszczyk;A. Ross;D. Herlyn;Z. Steplewski;H. Koprowski
中科院分区：
文献类型：
--
作者：
B. Atkinson;C. Ernst;B. Ghrist;M. Herlyn;M. Błaszczyk;A. Ross;D. Herlyn;Z. Steplewski;H. Koprowski
通过小鼠骨髓瘤细胞与黑素瘤免疫小鼠的淋巴细胞融合获得的杂交瘤的早期培养上清液，使用生物素：抗生物素蛋白免疫过氧化物酶程序，筛选其与黑素瘤细胞系的石蜡包埋细胞块的反应性。11个单克隆抗体，定义了几个新的黑色素瘤相关抗原。抗原包括中性糖脂、神经节苷脂、膜相关蛋白、胞质蛋白和强分泌蛋白。这些抗体检测能够承受组织固定和包埋程序的抗原，在固定的细胞系以及黑色素瘤活检中检测反应性。这些抗体可能为人类恶性黑色素瘤活检材料的诊断研究提供有力的工具。
Early culture supernatants from hybridomas that were obtained through fusions of mouse myeloma cells with lymphocytes of melanoma-immunized mice were screened for their reactivity with a paraffin-embedded cell block of a melanoma cell line, using a biotin:avidin immunoperoxidase procedure. Eleven monoclonal antibodies were derived that define several new melanoma-associated antigens. The antigens include a neutral glycolipid, gangliosides, membrane-associated proteins, cytosolic proteins, and strongly secreted proteins. These antibodies, which detect antigens that withstand tissue fixation and embedding procedures, were tested for reactivity in fixed cell lines, as well as in melanoma biopsies. These antibodies may provide powerful tools in diagnostic studies of human malignant melanoma biopsy material.