Lipopolysaccharide-evoked HSPA12B expression by activation of MAPK cascade in microglial cells of the spinal cord
Lipopolysaccharide-evoked HSPA12B expression by activation of MAPK cascade in microglial cells of the spinal cord
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通过激活脊髓小胶质细胞中的 MAPK 级联，脂多糖诱发 HSPA12B 表达
DOI：
10.1016/j.jns.2010.04.009
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发表时间：
2010-07-15
影响因子：
4.4
通讯作者：
Cheng, Chun
中科院分区：
文献类型：
--
作者：
Cui, Zhiming;Wang, Ping;Cheng, Chun
特别是热休克蛋白（HSP 70）的70 kDa家族，在细胞保护中起着至关重要的作用，并已在各种组织中检测到受到压力。HSPA 12 B是HSP 70家族的最新成员，但与HSP 70家族不同。本研究旨在阐明HSPA 12 B在脂多糖（LPS）诱导的成年大鼠神经炎症过程中的动态表达变化及定位。HSPA 12 B的表达强烈诱导活跃的小胶质细胞在发炎的脊髓。体外研究表明，HSPA 12 B的上调可能参与了LPS刺激后小胶质细胞的活化。HSPA 1/2 B表达的增加主要受MAPK-p38和ERK 1/2通路的调节，而SAPK/JNK通路的作用较小。总的来说，这些结果表明，HSPA 12 B可能是重要的宿主防御小胶质细胞介导的免疫反应。了解中枢神经系统的细胞信号通路，可能为中枢神经系统炎症反应和免疫反应的防治提供新的策略。皇冠版权所有（C）2010由爱思唯尔B. V.出版保留所有权利。
The 70-kDa family of heat shock proteins (HSP70), in particular, plays a vital role in cellular protection and has been detected in various tissues subject to stress. HSPA12B is the newest member of the HSP70 family but is distinct from the HSP70 family. In this study, we elucidated the dynamic expression changes and localization of HSPA12B in lipopolysaccharide (LPS)-induced neuroinflammatory processes in adult rats. HSPA12B expression was strongly induced in active microglial cells in inflamed spinal cord. In vitro studies indicated that the up-regulation of HSPA12B may be involved in the subsequent microglia activation following LPS challenge. The elevated HSPA12B expression was regulated by activation of MAPK-p38 and ERK1/2 pathways, less contribution of the SAPK/JNK pathway in microglial cells. Collectively, these results suggested HSPA12B may be important for host defense in microglia-mediated immune response. Understanding the cell signal pathway may provide a novel strategy against inflammatory and immune reaction in neuroinflammtion in CNS. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.